بررسی ارتباط بین فاصله ژنتیکی والدین با تظاهر هتروزیس در آفتابگردان تحت شرایط آبیاری نرمال و تنش کم‌آبی

نوع مقاله : علمی - پژوهشی

نویسنده

استادیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه مراغه، مراغه

چکیده

آفتابگردان از جمله محصولات مهم زراعی در دنیا می­باشد که تولید بذر هیبرید آن دارای اهمیت اقتصادی فراوانی است. از مهم­ترین مراحل تولید بذر هیبرید، آگاهی از هتروزیس و شناسایی ترکیبات والدینی برتر است که مرحله ­ای زمان­بر و پرهزینه می ­باشد. در این مطالعه ارتباط بین فاصله ژنتیکی بر­اساس نشانگر ریزماهواره با تظاهر هتروزیس در آفتابگردان بررسی گردید. تعداد پنج لاین آفتابگردان به­ صورت دی­آلل ۵ × ۵ یک­ طرفه تلاقی داده شد و سپس والدین و نتاج آنها در قالب طرح بلوک­ های کامل تصادفی با سه تکرار به­ طور جداگانه در شرایط محیطی آبیاری و تنش کم ­آبی در گلخانه کشت گردیدند. عملکرد دانه و محتوی نسبی آب برگ در هر تکرار اندازه ­گیری گردید. فاصله ژنتیکی جاکارد بین لاین­ های والدی با استفاده از ۳۱ مکان ریزماهواره­ای محاسبه گردید. فاصله ژنتیکی بین 0/58 تا 0/82 متغیر بود. براساس فواصل ژنتیکی محاسبه شده، ژنوتیپ ­های والدی مورد مطالعه در سه گروه قرار گرفتند. ارتباط فواصل ژنتیکی به­ دست آمده با ترکیب ­پذیری خصوصی و هتروزیس میانگین والدین برای صفات عملکرد دانه و محتوی آب نسبی برگ در هر یک از شرایط آبیاری، مورد آزمون قرار گرفت. نتایج نشان داد که نشانگر ریزماهواره می تواند به­ طور مؤثری در تشخیص چندشکلی و فاصله ژنتیکی بین لاین­های والدی مورد استفاده قرار گیرد. ارتباط فاصله ژنتیکی مبتنی بر نشانگر ریزماهواره با هتروزیس میانگین والدین و ترکیب­پذیری خصوصی برای صفات مورد ارزیابی معنی­ دار نبوده و فاقد کارایی لازم در پیش­ بینی هتروزیس می ­باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Relationship of Parental Genetic Distance with Heterosis Appearance in Sunflower under Well-Watered and Water-Stressed Status

نویسنده [English]

  • Hamid Hatami Maleki
Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh
چکیده [English]

Sunflower is one of the most important field crop over the world which it`s hybrid seed production is very important. Knowledge about heterosis and identification of superior parental combination is major step in producing hybrid seed that is time consuming process and expensive. Current study was aimed to investigate the relationship between genetic distances based on simple sequence repeat (SSR) markers with heterosis to provide the prediction of hybrids performance regarding to genetic distance. In present study, five sunflower inbred lines were crossed in 5×5 half diallel design and then, parental lines and their progenies were investigated using a randomized complete block design with three replications in both normal and water stress states in greenhouse. The Jaccard genetic distances among parental lines were calculated using 31SSR markers. Genetic distances were varied between 0.58 and 0.82. Based on calculated genetic distances, parental genotypes were located in three groups. Significant or nonsignificant relationships of obtained genetic distances with specific combining abilities and mid parent heterosis for seed yield and relative water content were tested in both states. Results revealed that microsatellite markers could be effectively used to fingerprinting and identification of genetic distance between parental lines. Result showed that relationship between genetic distance based on microsatellite marker with mid parent heterosis and specific combining ability for evaluated traits were not significant and have no efficiency in prediction of heterosis. 

کلیدواژه‌ها [English]

  • Microsatellite marker
  • Heterosis
  • Specific combining ability
  • Leaf relative water content
  • Grain yield
Beg, A., Rana, M. A. and Aslam, M. 1984. Sunflower production practices. Progressive Farming, 4: 14-19.
Bernardo, R. 1992. Relationship between single-cross performance and molecular marker heterozygosity. Theoretical and Applied Genetics, 83: 628-634.
Charcosset, A. M., Lefort-Buson, M. and Gallais, A. 1991. Relationship between heterosis and heterozygosity at marker loci: a theoretical computation. Theoretical and Applied Genetics, 81: 571-575.
Darvishzadeh, R. 2012. Phenotypic and molecular marker distance as a tool for prediction of heterosis and F1 performance in sunflower (Helianthus annuus L.) under well-watered and water-stressed conditions. Australian Journal of Crop Science,6: 732-738.
Darvishzadeh, R., Azizi, M., Hatami-Maleki, H., Bernousi, I., Abdollahi-Mandoulakani, B., Jafari, M. and Sarrafi, A. 2010. Molecular characterization and similarity relationships among sunflower (Helianthus annuus L.) Inbred lines using mapped simple sequence repeats. African Journal of Biotechnology, 9: 7280-7288.
Dellaporta, S. L. 1983. A plant minipreparation. Plant Molecular Biology Report, 1: 19-21.
Ebrahimi, A., Maury, P., Berger, M., Poormohammad-Kiani, S., Nabipour, A., Shariati, F., Grieu, P. and Sarrafi, A. 2008. QTL mapping of seed-quality traits in sunflower recombinant inbred lines under different water regimes. Genome, 51: 599-615.
García, B. F., Salinas, G. E., Pozo, O., Reyes, V. H., Ramírez, M. M., López, S. J. A., Aguirre, B. M. and Salazar, S. O. 2002. Estimation of genetic distances among green pepper (Capsicum annuum L.) lines using RAPD markers and its relationship with heterosis. Proceedings of the 16th International Pepper Conference Tampico. Tamaulipas, Mexico.
Gossal, S. S., Vasiljevic, L. and Brar, D. S. 1988. Plant biotechnology and sunflower improvement. Proceedings of 12th International Sunflower Conference. Novisad, Yugoslavia.
Gvozdenović, S., Saftić-Panković, D., Jocić, S. and Radić, V. 2009. Correlation between heterosis and genetic distance based on SSR markers in sunflower (Helianthus annuus L.). Journal of Agricultural Science, 54: 1-10.
Heidari-Sharif-Abadi, H. 2000. Plant, Drought and Dry Year. Ministry of Agriculture, Research Institute of Forests and Rangelands.
Hu, J., Seiler, G. and Kole, C. 2010. Genetics, genomics and breeding of sunflower. Routledge. USA. 360pp.
Kholghi, M., Darvishzadeh, R., Bernousi, I., Pirzad, A. and Laurentin, H. 2011. Assessment of genomic diversity among and within Iranian confectionery sunflower (Helianthus annuus L.) populations by using simple sequence repeat markers. Acta Agriculturae Scandinavica, Section B - Soil and Plant Science,62: 488-498.
Lariepe, A., Mangin, B., Jasson, S., Combes, V., Dumas, F., Jamin, P., Lariagon, C., Jolivot, D., Madur, D., Fievet, J., Gallais, A., Dubreuil, P., Charcosset, A. and Moreau, L. 2012. The genetic basis of heterosis: multiparental quantitative trait loci mapping reveals contrasted levels of apparent overdominance among traits of agronomical interest in Maize (Zea mays L.). Genetics, 190(2): 795-811.
Melchinger, A. E., Lee, M., Lamkey, K. R. and Woodman, W. L. 1990. Genetic diversity for restriction fragment length polymorphisms: relation to estimated genetic effects in maize inbreds. Crop Science, 30: 1033-1040.
Nabi pour, M., Farsi, M., Neamati, H. and Malekzadeh, S. 2012. Evaluation Genetic diversity of tomato genotypes using AFLP markers and its relationship with heterosis. Iranian Agricultural Research, 10: 354-360.
Nourjo, A., Abbasi, F., Bagaii Kia, M. and Jodaii, A. 2007. The effect of deficit irrigation on the quality and quantity of sugar beet in Miandoab region. Journal of Sugar Beet, 22: 53-66.
Poormohammad-Kiani, S., Grieu, P., Maury, P., Hewezi, T., Gentzbittel, L. and Sarrafi, A. 2007a. Genetic variability for physiological traits under drought conditions and differential expression of water stress-associated genes in sunflower (Helianthus annuus L.). Theoretical Applied Genetics,114: 193-207.
Poormohammad-Kiani, S., Talia, P., Maury, P., Grieu, P., Heinz, R., Perrault, A., Nishinakamasu, V., Hopp, E., Gentzbittel, L., Paniego, N. and Sarrafi, A. 2007b. Genetic analysis of plant water status and osmotic adjustment in recombinant inbred lines of sunflower under two water treatments. Plant Science, 172: 773-787.
Poormohammad-Kiani, S., Maury, P., Sarrafi, A. and Grieu, P. 2008. QTL analysis of chlorophyll fluorescence parameters in sunflower (Helianthus annuus L.) under well-watered and water-stressed conditions. Plant Science, 175: 565-573.
Poormohammad-Kiani, S., Maury, P., Nouri, L., Ykhlef, N., Grieu, P. and Sarrafi, A. 2009. QTL analysis of yield-related traits in sunflower under different water treatments. Plant Breeding, 128: 363-373.
Rauf, S., Sadaqat, H. A., Khan, I. A. and Ahmed, R. 2009. Genetic analysis of leaf hydraulics in sunflower (Helianthus annuus L.) under drought stress. Plant Soil and Environment, 55: 62-69.
Reif, J. C., Melchinger, A. E., Xia, X. C., Warburton, M. L., Hoisington, D. A., Vasal, S. K., Srinivasan, G., Bohn, M., Frisch, M. 2003. Genetic distance based on simple sequence repeats and heterosis in tropical maize population. Crop Science, 43: 1275-1282.
Ritchie, S. W. and Nguyen, H. T. 1990. Leaf water content and gas exchange parameters of two wheat genotypes differing in drought resistance. Crop Science, 30: 105-111.
Roy, D. 2000. Plant Breeding: Analysis and exploitation of variation. Alpha Science International LTD.
Sharp, G. L., Martin, J. M., Lanning, S. P., Blake, N. K., Brey, C. W., Sivamani, E., Qu, R. and Talbert, L. E. 2002. Field evaluation of transgenic and classical sources of wheat streak mosaic virus resistance. Crop Science, 42: 105-110.
Skoric, D., Jocic, S. and Molnar, I. 2000. General and specific combining abilities in sunflower. Proceeding of 15th international sunflower conference. Toulouse, France.
Tang, S., Yu, J. K., Slabaugh, M. B., Shintani, D. K. and Knapp, S. J. 2002. Simple sequence repeat map of the sunflower genome. Theoretical Applied Genetics, 105: 1124-1136.
Tezara, W., Mitchall, V., Driscoll, S. P. and Lawlor, D. W. 2002. Effects of water deficit and its interaction with CO2 supply on the biochemistry and physiology of photosynthesis in sunflower. Journal of Experimental Botany, 375: 1781-1791.
Teklewold, A. and Becker H. C. 2006. Comparison of phenotypic and molecular distances to predict heterosis and F1 performance in Ethiopian mustard (Brassica carinata A. Braun). Theoretical and Applied Genetic, 2: 752-759.
Torabi, S., Omidi, M., Shah-nejat-boshehri, A. A. and Gannadha, M. R. 2005. Comparison of genetic and morphological distance with heterosis based on RAPD markers in barley hybrids. Iranian Journal of Agricultural Sciences, 26: 493-501.
Woody, J. L., Severin, A. J., Bolon, Y. T., Joseph, B., Diers, B. W., Farmer, A. D., Weeks, N., Muehlbauer, G. J., Nelson, R. T., Grant, D., Specht, J. E., Graham, M. A., Cannon, S. B., May, G. D., Vance, C. P. and Shoemaker, R. C. 2011. Gene expression patterns are correlated with genomic and genic structure in soybean. Genome, 54: 10-8.
Xiao, J., Li, J., Yuan, L. and Tanksley, S. D. 1995. Dominance is the major genetic basis of heterosis in rice as revealed by QTL analysis using molecular markers. Genetics, 140: 745-54.
Zarei, L., Farshadfar, E., Haghparast, R., Rajabi, R. and Mohammadi, M. 2007. Evaluation of some indirect traits and indices to identify drought tolerance in bread wheat. Asian Journal of Plant Sciences, 6: 1204-1210.
Zhang, Q. F., Zhou, Z. Q., Yang, G. P., Xu, C. G., Liu, K. D. and Saghai-Maroof, M. A. 1996. Molecular marker heterozygosity and hybrid performance in indica and japonica rice. Theoretical and Applied Genetics, 93: 1218-1224.