تکثیر درون شیشه‏ ای انار رقم رباب نیریز با استفاده از قطعات گره‏ای ساقه

نوع مقاله : علمی - پژوهشی

نویسندگان

1 استادیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه اراک، اراک

2 استادیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه بوعلی‌سینا، همدان

3 استادیار بخش بیوتکنولوژی گیاهی، پژوهشکده بیوتکنولوژی، کرج

چکیده

در این تحقیق، یک روش سریع و کارآمد برای تکثیر درون­شیشه‏ای انار رقم رباب نیریز شرح داده می‏شود. اثر دو محیط­کشت WPM و MS، و تنظیم­کننده‏های رشد مختلف در ریزازدیادی این رقم بررسی شد. برای مرحله پرآوری، محیط­کشت دارای غلظت‏های مختلف کینتین (3/2، 7/4، 2/9 و 4/18 میکرومولار) همراه با 54/0 میکرومولار نفتالین استیک­اسید استفاده گردید. از نظر پرآوری، محیط­کشت WPM در مقایسه با MS کارآمدتر تشخیص داده شد. بهترین غلظت کینتین 2/9 میکرومولار بود که منجر به بیش­ترین طول شاخساره (93/3 سانتی­متر)، تعداد برگ (71/10) و تعداد گره (4) در ریزنمونه‏ها شد. محیط نصف غلظت WPM حاوی 4/5 میکرومولار NAA مؤثرترین محیط برای ریشه­زایی شاخساره‏ها بود. گیاهان ریشه­دار شده به­طور موفقیت­آمیزی سازگاری تدریجی شده و به خاک منتقل گردیدند. گیاهان ریز ازدیادی یافته از نظر مرفولوژیکی شبیه به هم بوده و صفات رویشی مشابه با گیاهان مادری نشان دادند.

کلیدواژه‌ها

عنوان مقاله [English]

In Vitro Propagation of Pomegranate (Punica granatum) cv. Rabab Neyriz Using Nodal Segments

نویسندگان [English]

  • babak valizade kaji 1
  • ahmad ershadi 2
  • masoud tohidfar 3
چکیده [English]

In this study, an efficient in vitro propagation method is described for Punica granatum cultivar ‘Rabab Neyriz’. The influence of two basal medium, WPM and MS, and different plant growth regulators was investigated on micropropagation of this cultivar. For proliferation stage, media supplemented with different concentrations (2.3, 4.7, 9.2 and 18.4 μM) of kinetin along with 0.54 μM NAA was used. On base of proliferation, WPM proved to be more efficient medium compared to MS. The best concentration of kinetin was 9.2 μM resulting in the highest shoot length (3.93 cm), leaf number (10.71) and node number (4). Half-strength WPM medium supplemented with 5.4 μM NAA was most effective for rooting of shoots. Rooted plantlets were successfully acclimatized and transferred into soil. The micropropagated plants were morphologically uniform and exhibited similar growth characteristics and vegetative morphology to the mother plants. 

کلیدواژه‌ها [English]

  • Pomegranate
  • In vitro propagation
  • Hormone

مراجع

Agarwal, S., Kanwar, K. and Sharma, D. R. 2004. Factors affecting secondary somatic embryogenesis and embryo maturation in Morus alba L. Scientia Horticulturae, 102: 359-368.
Chandrasekhar, T., Hussain, T., M. and Jayanand, B. 2005. In vitro micropropagation of Boswellia ovalifoliolata. Natural Center for Biotechnology Information, 60: 505-507.
Dalal, N. V. and Rai, V. R. 2004. In vitro propagation of Oroxylum indicum Vent.-a medicinally important forest tree. Journal of Forest Research, 9: 61-65.
De Klerk, G. J., Brugge, J. T. and Marinova, S. 1997. Effectiveness of indoleacetic acid, indolebutyric acid and naphthaleneacetic acid during adventitious root formation in vitro in Malus ‘Jork 9’. Plant Cell Tissue, and Organ Culture, 49: 39-44.
Heloir, M. C., Fournioux, J. C., Oziol, L. and Bessis, R. 1997. An improved procedure for the propagation in vitro of grapevine Vitis vinifera (cv. Pinot noir) using axillary bud cuttings. Plant Cell Tissue, and Organ Culture, 49: 95-108.
Jaidka, K. and Mehra, P. N. 1986. Morphogenesis in Punica granatum (Pomegranate). Canadian Journal of Botany, 64: 1644-1653.
Ibáñez, A., Valero, M. and Morte, A. 2005. Establishment and in vitro clonal propagation of the Spanish autochthonous table grapevine cultivar Napoleon-an improved system where proliferating cultures alternate with rooting ones. Anal. Biol, 27: 211-220.
Kahrizi, D., Arminian, A. and Masumi, A. A. 2011. In vitro plant breeding. Razi University Press, Kermanshah, Iran, pp 200.
Kanwar, K., Joseph, J. and Deepika, R. 2010. Comparison of in vitro regeneration pathways in Punica granatum L. Plant Cell Tissue, and Organ Culture, 100: 199-207.
Krishna, H. and Singh, N. K. 2007. Biotechnological advances in mango Mangifera indica L. and their future application in crop improvement-a review. Biotechnology Advances, 25: 223-243.
Kristiansen, K. 1992. Micropropagation of Ficus benjamina clones, plant cell tissue and organ culture, 28: 53-58.
Lloyd, G. B. and McCown, B. H. 1980. Commercially feasible micropropagation of mountain laurel, Kalmia latifolia by use of shoot tip culture. Combined Proceedings, International Plant Propagators' Society, 30: 421-427.
Moslemi, M., Zahravi, M. and Bakhshi Khanikic, G. H. 2010. Genetic diversity and population genetic structure of pomegranate (Punica granatum L.) in Iran using AFLP markers. Scientia Horticulture, 126: 441-447.
Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.
Murkute A.A., Patil, S. and Singh, S. K. 2004. In vitro regeneration in pomegranate cv. Ganesh from mature trees. Indian Journal of Horticulture, 61: 206-208.
Naik, S. K. and Chand, P. K. 2011. Tissue culture-mediated biotechnological intervention in pomegranate: a review. Plant Cell Reports, 30: 707-721.
Naik, S. K. and Chand, P. K. 2003. Silver nitrate and aminoethoxyvinylglycine promote in vitro adventitious shoot regeneration of pomegranate (Punica granatum L.). Journal of Plant Physiology, 160: 423-430.
Naik, S. K., Pattnaik, S. and Chand, P. K. 1999. In vitro propagation of pomegranate (Punica granatum L. cv. Ganesh) through axillary shoot proliferation from nodal segments of mature tree. Sci Hortic, 79: 175-183.
Naik, S. K., Pattnaik, S. and Chand, P. K. 2000. High frequency axillary shoot proliferation and plant regeneration from cotyledonary nodes of pomegranate (Punica granatum L.). Scientia Horticulturae,5: 261-270.
Nataraja, K. and Neelambika, G. K. 1996. Somatic embryogenesis and plantlet from petal cultures of pomegranate, Punica granatum L. Indian journal of experimental biology, 34: 719-721.
Perez-Tornero, O., Tallon, C. I. and Porras, I. 2010. An efficient protocol for micropropagation of lemon (Citrus limon) from mature nodal segments. Plant Cell Tissue, and Organ Culture, 100: 263-271.
Rai, M. K., Jaiswal, V. S. and Jaiswal, U. 2009. Shoot multiplication and plant regeneration of guava Psidium guajava L. from nodal explants of in vitro raised plantlets. JournalofFruitandOrnamental Plant Research, 17: 29-38.
Roubelakis - Angelakis, K. A. and Zivanovitc, S. B. 1991. A new culture medium for in vitro rhizogenesis of grapevine (Vitis spp.) genotypes. HortScience, 26: 1551-1553.
Shukla, S., Shukla, S. K. and Mishra, S. K. 2008. In vitro plant regeneration from seedling explants of Stereospermum personatum D. C.: a medicinal tree. Trees Structure and Function, 23: 409-413.
Singh, S. K. and Khawale, R. N. 2006. Plantlet regeneration from the nodal segments of pomegranate (Punica granatum L.) cv. Jyoti. In Kumar, A. and Roy, S. (eds) Plant biotechnology and its applications in tissue culture, IK International Pvt. Ltd., New Delhi, pp. 107-113.
Skiada, F. K., Grigoriadou, G. and Eleftheriou, E. P. 2010. Micropropagation of Vitis vinifera L. cv. ‘Malagouzia’ and ‘Xinomavro’. Central European JournalofBiology, 5(6): 839-852.
Tehranifar, A.,  Zarei, M., Nematia, Z., Esfandiyaria, B. and Vazifeshenasb, M. R. 2010. Investigation of physico-chemical properties and antioxidant activity of twenty Iranian pomegranate (Punica granatum L.) cultivars. Scientia Horticulturae, 126: 180-185.
Terakami, S., Matsuta, N., Yamamoto, T., Sugaya, S., Gemma, H. and Soejima, J. 2007. Agrobacterium-mediated transformation of the dwarf pomegranate (Punica granatum L. var. nana). Plant Cell Reports, 26: 1243-1251.
Tsukamoto, T., Gao, M., Negoro, K., Hanada, H., Tao, R., Kawabe, M. and Yonemori, K. 2007. Somatic embryogenesis and plant regeneration from immature cotyledons of Prunus mume ‘Nanko’. Acta Horticulturae, 738: 697-701.
 
 

فایل ها

هم رسانی

ارجاع به این مقاله

آمار