Genetic Diversity Among Iranian Cantaloupe Landraces
(Cucumis melo L.) Using Microsatellite Markers
Azam
Moaiedi nejad
Former M.Sc student, Department of Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamedan
author
Ahmad
Ershadi
Assistant professor, Department of Horticultural Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan
author
Jahangir
Abas kohpaigani
Assistant professor, Seed and Plant Improvement Institute, Karaj
author
Farshad
Dashti
Assistant professor, Department of Horticultural Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan
author
text
article
2012
per
The genetic diversity among 43 accessions of melon (C.melo L.), 41 from Cantaloupensis group alongside two from Indorus group, was assessed by variation at simple sequence repeats marker bands using 18 pair primers. The extracted genomic DNA was amplified with 12 pair primers and PCR products were separated on a DNA sequencing gels. A total of 98 alleles were identified with an average of 4.90 alleles per primer combination. Genetic distances among the accessions ranged from 0.0 for the most similar to 0.76 for the most-diverged ones. The mean GD (Nei's coefficient) among accessions was 0.219. The average of polymorphic information contents (PIC) for the 12 melon SSR markers was 0.542. CMCT134b, CMTC168, CMBR43 and CMAT141 loci had respectively the highest PICs, which could be used for further analysis. Genetic relationships among accessions were represented by a dendrogram based on similarity coefficient matrix with UPGMA method. Cluster analysis classified the accessions into 11 major groups. Cluster analysis indicated wide range of diversity across the Iranian and foreign accessions. The most distance was detected between Mahali e Darab and Amrikaie (76%). In general, poor relation was found between geographical and genetic diversity, whereas some relations was observed in cluster 2. The tetraploid accessions were mainly placed in the group 1. Principle component analysis had a very good co-ordination with dendrogram of genetic diversity. These results suggest that the SSR markers are valuable tools for identification and diversity analysis in cantaloupensis.
دوفصلنامه فن آوری زیستی در کشاورزی(علمی-پژوهشی)
دانشگاه بوعلی سینا
2476-6313
1
v.
1
no.
2012
1
8
https://ab.basu.ac.ir/article_108_6569ff059bee339a2d6f1348c46cd3d0.pdf
The Use of Nested PCR Method for Rapid Detection of Rosellinia necatrix Causal Agent of White Root Disease From Soil and Root
Seyed morteza
Mohammadi Meymand
Former MSc. Student of Plant Pathology, Department. of Plant Protection, College of Agriculture, Isfahan University of Technology
author
Bahram
Sharifnabi
Associate Professor of Plant Pathology, Department. of Plant Protection, College of Agriculture, Isfahan University of Technology
author
Massoud
Bahar
Associate Professor of Plant Pathology, Department. of Plant Protection, College of Agriculture, Isfahan University of Technology
author
text
article
2012
per
White root rot disease, caused by Rosellinia necatrix is one of the most important disease of fruit trees in Iran and worldwide. To detect the pathogen from soil and root, six infected soil samples were collected from cherry crown trees and two samples were collected as positive and negative control. DNA Extraction from eight soil samples including infected cherry roots by white cottony mycelium, roots infected by white mycelial fans and symptom less roots and two faba roots, with white cottony mycelium and symptom less root was performed. Detection of the Pathogen was done by two specific primer pairs R2، R8 and R7، R10 in Nested PCR reaction. The results showed the detection from six infected soil, positive control and in roots colonized by white cottony mycelium and roots by white mycelial fans and faba roots colonized by white cottony mycelium. No detection was achieved from symptom less roots. To verify the accuracy of the used detection method, ITS region was amplified and cloned in pTZ57R/T vector and sequenced. Comparison of the sequence showed %98.99 similarities to the recognized isolate of R. necatrix. According to our findings, it seems that Nested PCR method could be useful to rapid detection of R. necatrix from soil and root of plants in orchards.
دوفصلنامه فن آوری زیستی در کشاورزی(علمی-پژوهشی)
دانشگاه بوعلی سینا
2476-6313
1
v.
1
no.
2012
9
15
https://ab.basu.ac.ir/article_109_cdb1e4c4c39592f0a1254bcecfea55e9.pdf
Identification, Cloning and Characterization of a Thioredoxin h (VvTrxh10) Gene Isolated from Grape (Vitis vinifera L.) cv. Yaquti Berry Tissue
Seyed Sharafeldin
Mussavi
Graduate in MSc of Agricultural Biotechnology, Department of Agricultural Biotechnology, Imam Khomeini International University, Qazvin, IR of Iran
author
Raheem
Haddad
Assistant Professor, Department of Agricultural Biotechnology, Imam Khomeini International University, Qazvin, IR of Iran
author
ghasem-ali
garousi
Assistant Professor, Department of Agricultural Biotechnology, Imam Khomeini International University, Qazvin, IR of Iran, P. O. Box: 34149-288 Tel: 0281- 8371165, Fax: 3780073.
author
Ramin
Hosseini
Assistant Professor, Department of Agricultural Biotechnology, Imam Khomeini International University, Qazvin, IR of Iran, P. O. Box: 34149-288 Tel: 0281- 8371165, Fax: 3780073
author
text
article
2012
per
Total RNA was extracted from grape (Vitis vinifera L.) cv. Yaquti berry tissue to characterize a thioredoxin h gene (VvTrxh10). A cDNA library was synthesized using reverse transcription polymerase chain reaction (RT-PCR). Then, the VvTrxh10 gene was amplified, isolated and cloned in a pUC19 vector plasmid. Nucleotide sequence analysis revealed that the cloned cDNA expressed thioredoxin and contained a single open reading frame of 345 bp encoding a protein of 114 amino acid residues. Predicted protein sequence analysis showed that this gene contains a nongeneral catalic site RCGLC, characteristic tryptophan (W) and potential structural motif involving cell-to-cell taransfer (MAEE) in N-terminal. Phylogenetic and alignment studies revealed that such isoform belongs to the subgroup I from h thioredoxins group. Moreovere, relevant predicted protein exhibited a high similarity with the other plant thioredoxins h gene in the NCBI gene bank.
دوفصلنامه فن آوری زیستی در کشاورزی(علمی-پژوهشی)
دانشگاه بوعلی سینا
2476-6313
1
v.
1
no.
2012
17
26
https://ab.basu.ac.ir/article_110_c6e9344239d70949c870d39876964e89.pdf
Effect of Cold Pretreatment and Period of Preconditioning Inoculation on Transformation Frequency in Rapeseed (Brassica napus L.)
Danial
Kahrizi
Assistant Professor, Agronomy and Plant Breeding Department, Faculty of Agriculture, Razi University, Kermanshah
author
Alireza
Zebarjadi
Assistant Professor, Agronomy and Plant Breeding Department, Faculty of Agriculture, Razi University, Kermanshah
author
Ali Hatef
Salmanian
Associate Professor, National Institute for Genetic Engineering and Biotechnology,Tehran
author
text
article
2012
per
Genetic engineering in rapeseed will lead to the generation of plant varieties possessing more agriculturally and economically viable genetic traits. The most gene transformations to rapeseed have been done through Agrobacterium tumeifaciens method. Agrobacterium mediated transformation is depend on many parameters that must be optimized. The purpose of this study was to determine the effect of cold pretreatment (control and 12 h) among a 5 day old-plantlets with preconditioning period (0, 24 and 48 h) and inoculation period of explants in Agrobacterium solutions (2, 10, 20 and 40 s) on the gus reporter gene transformation frequency in Rapeseed. The experimental design was factorial on basis of completely randomized design (CRD) with four replications. The gene was transferred to a commercial cultivar rapeseed (PF-7045-91) via A. tumeifaciens (LBA4404 strain) mediated transformation method. Moreover, using PCR technique and gus assay, the presence and expression of genes in plants were confirmed. Statistical analysis revealed that there was no significant difference between cold pretreatment and control group. Moreover, the all interaction effects were not significant. Results also demonstrated that there was a significant difference among preconditioning and inoculation period levels for transformation efficiency. The highest effect on transformation efficiency was observed through 24 and 48 h preconditioning periods (with same effects and means 24.21 and 23.55%) and 10, 20 and 40 s inoculation periods (with same effects and means 20.50, 20.63 and 20.54%) respectively.
دوفصلنامه فن آوری زیستی در کشاورزی(علمی-پژوهشی)
دانشگاه بوعلی سینا
2476-6313
1
v.
1
no.
2012
27
34
https://ab.basu.ac.ir/article_111_a411a09e5e61683e5323069e4b9b30ae.pdf
Study on the Genetic Polymorphisms of Candidate Genes (Calpastatin and BoLA) in Sistani Cattle Using PCR-RFLP
mehdi
Khosravi
M.Sc. Graduated of Animal Science, Yong Researchers Club Azad University of Kashmar, Kashmar
author
Mohsen
Fakhar Kazemi
M.Sc. Graduated of Animal Science, Yong Researchers Club Azad University of Kashmar, Kashmar
author
Amir
Mohammadi
M.Sc. Graduated of Animal Science of Ferdowsi University of Mashhad
author
Mohammad Reza
Nassiry
Assistant Professor of Ferdowsi University of Mashhad
author
text
article
2012
per
Selection based on molecular markers is one of the new methods that may improve progress and accuracy of selection in animal breeding programs. Candidate genes of Calpastatin and (BoLA Bovine Leucocyte Antigen DRB3) were studied. Calpastatin gene is the candidate gene investigating growth rate and meat tenderness and DRB3 is extensively evaluated as a candidate marker for associations with various bovine diseases and immunological traits. Blood samples were taken from 89 Sistani cows in Zehak Research Institute. Genomic Extraction and PCR reaction were done for detect genomic variation of Bovine Leucocyte Antigen and Calpastatin. Amplicons were digested with restriction enzymes MspI for Calpastatin and RsaI, HaeIII and BstYI for BoLA DRB3 genes, respectively. Allelic frequencies for Calpastatin were M=0.764 and N=0.236, respectively. χ2 test showed That population is in hardy-weinberg equliberium. In this studies 19 alleles were identified in the studied Sistani herd. Allelic frequencies ranged from 0.1 to 0.22 in the Sistani population. The most frequent alleles were *8 and *34. These data provide evidence that Sistani breed have a variability, which opens interesting prospects for future selection programs, especially marker-assistant selection.
دوفصلنامه فن آوری زیستی در کشاورزی(علمی-پژوهشی)
دانشگاه بوعلی سینا
2476-6313
1
v.
1
no.
2012
35
42
https://ab.basu.ac.ir/article_112_9a74ab9e83b1187dea328eecf853babd.pdf
Characterization of Ethanol Inducible Gene Expression System in Transgenic Sugar Beet Calli Derived From Stomatal Guard Cells
Asghar
Mirzaie Asl
Ph.D student, Plant Breeding Department, Tarbiat Modares University, Tehran
author
Ahmad
Moieni
Associate Professor, Plant Breeding Department, Tarbiat Modares University, Tehran
author
Ali Hatef
Salmanian
Associate Professor, National Institute for Genetic Engineering and Biotechnology, Tehran
author
Mokhtar
Jalali Javaran
Associate Professor, Plant Breeding Department, Tarbiat Modares University, Tehran
author
Leila
Khodaei
PhD student, College of Agriculture and Natural Resources, Department of Agronomy and Plant Breeding, Tehran University
author
text
article
2012
per
Controlled gene expression of transgenic plants is one of the main aim of genetic manipulations. This control can be done in transcriptional level. Ethanol inducible promoter system is suitable for external regulation of gene expression. In this study, GUS gene expression under the control of ethanol inducible promoter was evaluated in calli derived from sugar beet stomatal guard cell protoplasts. Protoplasts of stomatal guard cells from sugar beet leaves were isolated and calli derived from these protoplasts, were infected with Agrobacterium harboring GUS gene under the control of ethanol inducible promoter. Transformed calli were treated with ethanol and histochemical GUS assay carried out before and after treatment of calli with ethanol. Some transformed calli showed high level expression of GUS gene after treatment with ethanol. These calli didn’t show any GUS gene expression before applying ethanol. The level of GUS expression after ethanol induction was comparable to constitutive GUS gene expression mediated by CaMV 35S promoter. Furthermore high level GUS expression in these calli demonstrated the activity of this system in sugar beet.
دوفصلنامه فن آوری زیستی در کشاورزی(علمی-پژوهشی)
دانشگاه بوعلی سینا
2476-6313
1
v.
1
no.
2012
43
49
https://ab.basu.ac.ir/article_113_b9758f494d6f5b6d0a04b598182e03c1.pdf