1
Graduate in MSc of Agricultural Biotechnology, Department of Agricultural Biotechnology, Imam Khomeini International University, Qazvin, IR of Iran
2
Assistant Professor, Department of Agricultural Biotechnology, Imam Khomeini International University, Qazvin, IR of Iran
3
Assistant Professor, Department of Agricultural Biotechnology, Imam Khomeini International University, Qazvin, IR of Iran, P. O. Box: 34149-288 Tel: 0281- 8371165, Fax: 3780073.
4
Assistant Professor, Department of Agricultural Biotechnology, Imam Khomeini International University, Qazvin, IR of Iran, P. O. Box: 34149-288 Tel: 0281- 8371165, Fax: 3780073
Abstract
Total RNA was extracted from grape (Vitis vinifera L.) cv. Yaquti berry tissue to characterize a thioredoxin h gene (VvTrxh10). A cDNA library was synthesized using reverse transcription polymerase chain reaction (RT-PCR). Then, the VvTrxh10 gene was amplified, isolated and cloned in a pUC19 vector plasmid. Nucleotide sequence analysis revealed that the cloned cDNA expressed thioredoxin and contained a single open reading frame of 345 bp encoding a protein of 114 amino acid residues. Predicted protein sequence analysis showed that this gene contains a nongeneral catalic site RCGLC, characteristic tryptophan (W) and potential structural motif involving cell-to-cell taransfer (MAEE) in N-terminal. Phylogenetic and alignment studies revealed that such isoform belongs to the subgroup I from h thioredoxins group. Moreovere, relevant predicted protein exhibited a high similarity with the other plant thioredoxins h gene in the NCBI gene bank.
Mussavi, S. S., Haddad, R., garousi, G., & Hosseini, R. (2012). Identification, Cloning and Characterization of a Thioredoxin h (VvTrxh10) Gene Isolated from Grape (Vitis vinifera L.) cv. Yaquti Berry Tissue. Agricultural Biotechnology, 1(1), 17-26.
MLA
Mussavi, S. S., Haddad, R., garousi, G., & Hosseini, R. "Identification, Cloning and Characterization of a Thioredoxin h (VvTrxh10) Gene Isolated from Grape (Vitis vinifera L.) cv. Yaquti Berry Tissue", Agricultural Biotechnology, 1, 1, 2012, 17-26.
HARVARD
Mussavi S. S., Haddad R., garousi G., Hosseini R. (2012). 'Identification, Cloning and Characterization of a Thioredoxin h (VvTrxh10) Gene Isolated from Grape (Vitis vinifera L.) cv. Yaquti Berry Tissue', Agricultural Biotechnology, 1(1), pp. 17-26.
CHICAGO
S. S. Mussavi, R. Haddad, G. garousi & R. Hosseini, "Identification, Cloning and Characterization of a Thioredoxin h (VvTrxh10) Gene Isolated from Grape (Vitis vinifera L.) cv. Yaquti Berry Tissue," Agricultural Biotechnology, 1 1 (2012): 17-26,
VANCOUVER
Mussavi S. S., Haddad R., garousi G., Hosseini R. Identification, Cloning and Characterization of a Thioredoxin h (VvTrxh10) Gene Isolated from Grape (Vitis vinifera L.) cv. Yaquti Berry Tissue. Agric. Biotechnol. 2012;1(1):17-26 (In Persian).
