Identification of Oil Degrading Bacteria from Poldokhtar Polluted Areas and Investigation of Factors Affecting Their Degradation Performance

Document Type : research

Authors

1 Plant Pathologists Graduate Student, Department of Plant Protection,Faculty of Agriculture, University of Lorestan, Khoramabad

2 Assistant Professor, Department of Plant Protection, Faculty of Agriculture, University of Lorestan, Khoramabad

3 Coach, Department of Plant Protection, Faculty of Agriculture, University of Lorestan, Khoramabad

Abstract

Pollution of natural ecosystems by petroleum compounds have been more considered because of their high persistence in environment and adverse impacts on human health. Nowadays, a wide range of physical, chemical, thermal and bioremediation methods are employed in order to remediate contaminated sites. Bioremediation technology is based on use the natural or engineered microorganisms for reclamation of contaminated sites and environmental conservation. The current study was carried out in order to isolate and characterize the oil degrading native bacteria from Tange Fani Poldokhtar region, Lorestan province, Iran. Soil and water of contaminated sites were sampled and after transfer to the laboratory, samples were cultivated on the nutrient agar, nutrient broth and 2xYP media. After purification, bacterial strains were identified on the basis of phenotypic-biochemical traits and sequence of 16S rDNA gene as Pseudomonas aeruginosa, Acinetobacter junii, Acinetobacter baumannii, Delftia tsuruhatensis, Sphingobacterium multivorum, Stenotrophomonas acidaminiphila and Comamonas koreensis. Furthermore, the bacterial strains were evaluated regarding biofilm formation, siderophore production and swarming motion, which the highest amount of biofilm formation and swarming motion was belonged to P. aeruginosa. In this study, bacterium Comamonas koreensis for the first time as oil degrading introduced.

Keywords

References

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