Detection of Trichothecene Chemotypes of Fusarium graminearum Isolates in Wheats of Sistan va Baluchestan Province

Document Type : research

Authors

1 M.Sc. Student of Plant Pathology, Department of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol

2 Assistant Professor of Plant Biotechnology, Department of Plant Protection and Institute of Plant Biotechnology, Faculty of Agriculture, University of Zabol, Zabol

3 Assistant Professor of Molecular Genetic, Faculty of medicine, University of medical science of Zabol, Zabol

Abstract

Fusarium graminearum is one of the most important causes of wheat scab in different part of the world. This fungus is able to produce widespread Trichothecene mycotoxin such as Nivalenol and Deoxynivalenol which are harmful for both human and animals. The wheat ear samples were collected from wheat fields in Sistan and Baluchestan province. Sampling was performed during 2011-2012. The fungal isolates were separated, purified , then identified using various keys for Fusarium spp. and 293 isolates of Fusarium species belonging to eight species were isolated and identified. Among the identified isolates, F. graminearum showed the highest frequency (68.8%) compared to other species.When the isolates of F. graminearum were morphologically identified, 168 isolates were chosen using species-specific primer pairs (Fg16F/Fg16R). In these isolates, the presence of three genes: Tri13, Tri5 and Tri7 were detected by using PCR and specific primers for the genes. The two NIV and DON chemotype was detected among isolates of F. graminearumusing specific primers. The results of PCR reaction with specific primers showed that all tested isolates possess the genes involved in production of trichothecene. Therefore, the detection of genes involved in trichothecene production using species-specific primers to determine Fusarium isolates producer trichothecene could alternative the chemical expensive and time procedure.

References

Abedi-Tizaki, M. and Sabbagh, S. K. 2012. Morphological and molecular identification of Fusarium head blight isolates from wheat in North of Iran. Australian Journal of Crop Science, 6: 1356-1361.
Carter, J. P., Rezanoor, H. N., Holden, D. Desjardins, A. E., Plattner, R. D., and Nicholson, P. 2002. Variation in pathogenicity associated with the genetic diversity of Fusarium graminearum. European Journal of Plant Pathology, 108: 573-583.
Chandler, E. A., Simpson, D. R., Thomsett, M. A. and Nicholson, P. 2003. Development of PCR assays to Tri7 and Tri13 trichothecene biosynthetic genes, and characterisation of chemotypes of Fusarium graminearum, Fusarium culmorum and Fusarium cerealis. Physiological and Molecular Plant Pathology, 62: 355-367.
Desjardins, A. E., Proctor, R. H., Bai, G., McCormick, S. P., Shaner, G., Buechley, G. and Hohn, T. M. 1996. Reduced virulence of trichothecene-nonproducing mutants of Gibberella zeae in wheat field tests. Molecular Plant Microbe Interactions, 9: 775-781.
Edwards, S. G., Pirgozliev, S. R., Hare, M. C. and Jenkinson, P. 2001. Quantification of trichothecene-producing Fusarium species in harvested grain by competitive PCR To determine efficacies of fungicides against fusarium head blight of winter wheat. Applied and environmental microbiology, 67: 1575-1580.
Fekete, C., Logrieco, A., Giczey, G. and Hornok, L. 1997. Screening of fungi for the presence of the trichodiene synthase encoding sequence by hybridization to the Tri5 gene cloned from Fusarium poae. Mycopathologia, 138:91-97.
Foroud, N. A. and Eudes, F. 2009. Trichothecenes in cereal grains. International journal of molecular sciences, 10: 147-173.
Ji, L., Cao, K., Hu, T. and Wang, S. 2007. Determination of deoxynivalenol and nivalenol chemotypes of Fusarium graminearum isolates from China by PCR assay. Journal of Phytopathology, 155: 505-512.
Jones, RK. 2000. Assessments of Fusarium head blight of wheat and barley in response to fungicide treatment. Plant Disease, 84:1021-1030.
Kimura, M., Tokai, T., O’Donnell, K., Ward, T. J., Fujimura, M., Hamamoto, H., Shibata, T. and Yamaguchi, I. 2003. The trichothecene biosynthesis gene cluster of Fusarium graminearum F15 contains a limited number of essential pathway genes and expressed non-essential genes. FEBS letters, 539:105-110.
Lee, T., Han, Y. K., Kim, K. H., Yun, S. H. and Lee, Y. W. 2002. Tri13 and Tri7 determine deoxynivalenol-and nivHalenol-producing chemotypes of Gibberella zeae. Applied and Environmental Microbiology, 68: 2148-2154.
Lee, T., Oh, D. W., Kim, H. S., Lee, J., Kim, Y. H., Yun, S. H. and Lee, Y. W. 2001. Identification of deoxynivalenol-and nivalenol-producing chemotypes of Gibberella zeae by using PCR. Applied and environmental microbiology, 67: 2966-2972.
Logrieco, A., Bottalico, A., Mulé, G., Moretti, A. and Perrone, G. 2003. Epidemiology of toxigenic fungi and their associated mycotoxins for some Mediterranean crops. European Journal of Plant Pathology, 109: 645-667.
Merhej, J. Richard-Forget, F. and Barreau, C. 2011. Regulation of trichothecene biosynthesis in Fusarium: recent advances and new insights. Applied Microbiology and Biotechnology, 91: 519-528.
Mule, G., Gonzalez-Jaen, M. T., Hornok, L., Nicholson, P. and Waalwijk, C. 2005. Advances in molecular diagnosis of toxigenic Fusarium species: A Review. Food Additives and Contaminants, 22:316-323.
Nelson, P. E., Toussoun, T. A. and Marasa, W. 1983. Fusarium Species: An IllustratedManualfor Identification. University Park, PA, Pennsylvania State University Press, 193pp.
Nicholson, P., Rezanoor, H, Simpson, D., Joyce, D. 1997. Differentiation and quantification of the cereal eyespot fungi Tapesia yallundae and Tapesia acuformis using a PCR assay. Plant Pathology, 46: 842-856
 Nicholson, P., Simpson, D. R., Weston, G., Rezanoor, H. N., Lees, A. K., Parry, D. W. and Joyce, D. 1998. detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays. Physiological and Molecular Plant Pathology, 53: 17-37.
Nicholson P., Simpson D.R., Wilson, A. H., Chandler E., Thomsett M. 2004. Detection and Differentiation of trichothecene and Enniatin-Producing Fusarium species on small-grain cereals. European Journal of Plant Pathology, 110: 503-514.
Niessen, L., Schmidt, H. and Vogel, R. F. 2004. The use of tri5 gene sequences for PCR detection and taxonomy of trichothecene-producing species in the Fusarium section Sporotrichiella. International Journal of Food Microbiology, 95: 305-319.
Nirenberg H. I. 1981. A simplified method for identifying Fusarium spp. occurring on wheat. Canadian Journal of Botany, 59: 1599-1609.
Omurtag, G. Z. 2008. Fumonisins, trichothecenes and zearalenone in cereals. International Journal of Molecular Sciences, 9: 2062-2090.
Parry, D. W., Jenkinson, P. and McLeod, L. 2007. Fusarium ear blight (scab) in small grain cereals-a review. Plant Pathology, 44: 207-238.
Talas, F., Parzies, H. K. and Miedaner, T. 2011. Diversity in genetic structure and chemotype composition of Fusarium graminearum sensu stricto populations causing wheat head blight in individual fields in Germany. European Journal of Plant Pathology, 131: 39-48.
Toth, B., Mesterhazy, A., Nicholson, P., Teren, J. and Varga, J. 2004. Mycotoxin production and molecular variability of European and American isolates of Fusarium culmorum, Molecular Diversity and PCR-detection of Toxigenic Fusarium Species and Ochratoxigenic Fungi, Springer, 587-599pp.
Waalwijk, C., Kastelein, P., de Vries, I., Kerényi, Z., van der Lee, T., Hesselink, T., Köhl, J. and Kema, G. 2003. Major changes in Fusarium spp. in wheat in the Netherlands. European Journal of Plant Pathology, 109: 743-754.
Yörük, E. and Albayrak, G. 2012. Chemotyping of Fusarium graminearum and F. culmorum Isolates from Turkey by PCR Assay. Mycopathologia, 173: 53-61.
 
 
 
 
 
 
 
 
 
 
 
 
 

Files

Share

How to cite

Statistics