Detection of Trichothecene Chemotypes of Fusarium graminearum Isolates in Wheats of Sistan va Baluchestan Province

Document Type : research

Authors

1 M.Sc. Student of Plant Pathology, Department of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol

2 Assistant Professor of Plant Biotechnology, Department of Plant Protection and Institute of Plant Biotechnology, Faculty of Agriculture, University of Zabol, Zabol

3 Assistant Professor of Molecular Genetic, Faculty of medicine, University of medical science of Zabol, Zabol

Abstract

Fusarium graminearum is one of the most important causes of wheat scab in different part of the world. This fungus is able to produce widespread Trichothecene mycotoxin such as Nivalenol and Deoxynivalenol which are harmful for both human and animals. The wheat ear samples were collected from wheat fields in Sistan and Baluchestan province. Sampling was performed during 2011-2012. The fungal isolates were separated, purified , then identified using various keys for Fusarium spp. and 293 isolates of Fusarium species belonging to eight species were isolated and identified. Among the identified isolates, F. graminearum showed the highest frequency (68.8%) compared to other species.When the isolates of F. graminearum were morphologically identified, 168 isolates were chosen using species-specific primer pairs (Fg16F/Fg16R). In these isolates, the presence of three genes: Tri13, Tri5 and Tri7 were detected by using PCR and specific primers for the genes. The two NIV and DON chemotype was detected among isolates of F. graminearumusing specific primers. The results of PCR reaction with specific primers showed that all tested isolates possess the genes involved in production of trichothecene. Therefore, the detection of genes involved in trichothecene production using species-specific primers to determine Fusarium isolates producer trichothecene could alternative the chemical expensive and time procedure.

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