Agricultural Biotechnology

Agricultural Biotechnology

Identification, Cloning and Characterization of a Thioredoxin h (VvTrxh10) Gene Isolated from Grape (Vitis vinifera L.) cv. Yaquti Berry Tissue

Document Type : Research Paper

Authors
Seyed Sharafeldin Mussavi 1
Raheem Haddad 2
ghasem-ali garousi 3
Ramin Hosseini 4
1 Graduate in MSc of Agricultural Biotechnology, Department of Agricultural Biotechnology, Imam Khomeini International University, Qazvin, IR of Iran
2 Assistant Professor, Department of Agricultural Biotechnology, Imam Khomeini International University, Qazvin, IR of Iran
3 Assistant Professor, Department of Agricultural Biotechnology, Imam Khomeini International University, Qazvin, IR of Iran, P. O. Box: 34149-288 Tel: 0281- 8371165, Fax: 3780073.
4 Assistant Professor, Department of Agricultural Biotechnology, Imam Khomeini International University, Qazvin, IR of Iran, P. O. Box: 34149-288 Tel: 0281- 8371165, Fax: 3780073
Abstract
Total RNA was extracted from grape (Vitis vinifera L.) cv. Yaquti berry tissue to characterize a thioredoxin h gene (VvTrxh10). A cDNA library was synthesized using reverse transcription polymerase chain reaction (RT-PCR). Then, the VvTrxh10 gene was amplified, isolated and cloned in a pUC19 vector plasmid. Nucleotide sequence analysis revealed that the cloned cDNA expressed thioredoxin and contained a single open reading frame of 345 bp encoding a protein of 114 amino acid residues. Predicted protein sequence analysis showed that this gene contains a nongeneral catalic site RCGLC, characteristic tryptophan (W) and potential structural motif involving cell-to-cell taransfer (MAEE) in N-terminal. Phylogenetic and alignment studies revealed that such isoform belongs to the subgroup I from h thioredoxins group. Moreovere, relevant predicted protein exhibited a high similarity with the other plant thioredoxins h gene in the NCBI gene bank.
Keywords
Grapevine
Thioredoxin h
Cloning
Sequence analysis
Yaquti cultivar