Effect of Cultivar and Density of Cultured Cotyledons on Shoot Regeneration in Rapeseed (Brassica napus L.)

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Biotechnology Research for Drought Resistance, Faculty of Agriculture, Razi University, Kermanshah

2 Associate Professor. National Institute for Genetic Engineering and Biotechnology,Tehran

Abstract

The objective of the present research was to study the effect of genotypes (2 commercial cultivars of repeseed, PF-7045-91 and SLM-046) and explant densities (15 and 30 cotyledons in10 cmin diometer Petri dishes) on shoot regeneration. Results showed that there were significant differences between rapeseed cultivars and explant densities, but no significant interaction effect was observed between investigated parameters. The cultivar PF-7045-91 was better regenerated with average of 85% regenerated cotyledons than the other one with 62%. Higher percentage of regeneration (87%) was also recorded for treatment with 15 cotyledons in Petri dish rather than 30 cotyledons.
 

Keywords

Files

Share

How to cite

Statistics