Agricultural Biotechnology

Agricultural Biotechnology

Isolation and Characterization of a Chitinase Gene Exhibiting Antifungal Activity Against Fusarium solani f. sp. melongenae from an Iranian Streptomyces strain

Document Type : Research Paper

Authors
Azam Baharlouei 1
Gholam Reza Sharifi-sirchi 2
Gholam Hossein Shahidi Bonjar 3
1 Graduate Student , Department of Agricultural Biotechnology, Faculty of Agricultur, Shahid Bahonar University of Kerman
2 Assistant Professor, Department of Agricultural Biotechnology, Faculty of Agricultur, Shahid Bahonar University of Kerman
3 Professor, Department of Plant Pathology, Faculty of Agricultural Sciences, Shahid Bahonar University of Kerman
Abstract
Fusarium solani f. sp. melongenae, the causal agent of egg plant wilt disease, has a wide host range which causes significant reduction in yield of eggplant (Solanum melongena). In a survey for finding biocontrol agents, 110 Actinomycetes strains were isolated from agricultural soils of Kerman province. Amony isolated strains 18 strain showed high level of chitinase activity Streptomyces griseus. Streptomyces isolate 401 significantly reduced the incidence of disease. Some biological and physiological characteristics of this isolate were investigated under green house condition. Moreover, the genomic DNA of this isolate was extracted by useing of CTAB method and a full chitinase gene (876 bp) was cloned and sequenced. The results showed high similarity between obrouned scyuence and seyuence of chitinasegene 19 fmily from.
Keywords
Biological control
Streptomyces
Chitinase
Fusarium solani
Agricultural Biotechnology
Volume 1, Issue 2 - Serial Number 2
December 2010
Pages 39-47