Effects of Hormon and Kanamycine on Calli of Medicinal Plant (Papaver somniferum L.)

Document Type : Research Paper

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Abstract

Opium poppy (Papaver somniferum) is one of the oldest and most important medicinal plants due to biosynthesis and storage of Benzylisoquinoline alkaloids. For more biotechnology exploitation of this plant, optimization of tissue culture and genetic transformation is necessary. Among different factors affecting on genetic transformation, selectable marker and it's interaction with other factors is important. In order to optimization of gene transformation by agrobacterium to the plant, three separate experiments were designed for callus induction from hypocotyl, cotyledon and root explants. The factorial experiments were designed for callus induction with two factors based on completely randomized design with 8 replicates. First factor was basal medium (B5 and MS) and second factor was plant growth regulation (1mg/l 2, 4-D and 0.5 2,4-D mg/l + 0.5 BA mg/l ). Analysis of variance and mean comparisons revealed that the highest percentage of callugenesis was observed in B5 Medium. At somatic embryogenesis stage, factorial experiments (with tree factors including medium, hormones and kanamycin) were performed based on completely randomized design with 8 replicates. Results indicated that the most embrygenesis and regeneration of somatic embryonic obtained from hypocotyl explants on B5 medium supplemented with 1 mg/l 2,4-D and 25 mg/l Kanamaycin.

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References

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