Transcriptom Analysis of Induced Genes in Response to Salt Stress in the Halophyte Aeluropus littoralis

Document Type : Research Paper

Authors

1 MSc of Plant Breeding, Genetic and Agricultural Biotechnology Institute of Tabarestan, University of Agriculture and Natural Resources of Sari

2 Prophosser of Genetic and Agricultural Biotechnology Institute of Tabarestan, University of Agriculture and Natural Resources of Sari

3 Professor assistant of Department of Biotechnology, Faculty of New Technologies and Energy Engineering, Shahid Beheshti University, Tehran

4 Genetic and Agricultural Biotechnology Institute of Tabarestan, University of Agriculture and Natural Resources of Sari

Abstract

Soil salinity limits crop production and affects developmental aspects at first Leading to reduced plant production. In this study, gene expression analysis based on cDNA-AFLP technique was used to compare the expression profiles of NaCl stress at three levels, 1 week after subculture: 0 (control), 200mM and 400mM, in roots of Aeluropus littoralis which is the closest family to cereal. Among 32 isolated ESTs, 25 ESTs were obtained with the average length of 250 bp. The nucleotide sequences were compared with those in the GenBank database. Approximately 80% of the ESTs show homology to nucleotide or amino acid sequences in the GenBank database and 7 ESTs show no significant similarity in the GenBank database which considered as novel genes. Finally, 25 ESTs were recorded in NCBI database which are included potassium transporter, ribosomal protein, NADH dehydrogenase and golgin. The result of this research is very important to understand molecular basis and resistance mechanisms of drought stress for breeding and genetic engineering to improve crop resistance against stress and the production of resistant plants.

Keywords

References

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