Genetic Diversity Survey of Iranian Native Apples (Malus× domestica) Genotypes Using SSR Markers

Document Type : Research Paper

Authors

1 Former MSc student, Department of Horticulture, Urmia University, Iran

2 Assistant Professor Respectively, Department of Horticulture, Urmia University, Iran

Abstract

The genetic diversity of 44 apple genotypes from all over part ofIranwas evaluated using 16 pairs of SSR primers. In Total, 45 alleles were detected. The number of alleles per locus varied from 2 to 5 with an average of 2.8. The average number of effective alleles was 2.2, the average of expected and observed heterozygosity were 0.52 and 0.45, respectively. According to Chi-square test, population was out of Hardy–Weinberg equilibrium. The Polymorphism Information Content (PIC) ranged from 0.18 to 0.76 implying the high detecting potential of some studied primers. Based on Jaccard’s coefficients similarity matrix for all studied apple genotypes, the highest similarity was observed between "Salmas4" and "Dirras-eMashhad" and the The lowest one was found between "Meshki-eDamavand2" and "Sifeshirin". Cluster analysis by using UPGMA algorithm revealed two main groups. Considering to long juvenile period in fruit trees such as apple, it seems that the application of markers with high degree of polymorphism and linked to appropriate trait would be useful in apple genetic diversity studies and breeding activities.

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