Evaluation of Rapeseed Genotypes Response to Drought Stress via Callus Culture

Document Type : Research Paper

Authors

1 M. Sc. Student, Department of Plant Breeding and Agronomy, Faculty of Agriculture, Razi University, Kermanshah

2 Assistant Professor respectively, Department of Plant Breeding and Agronomy, Faculty of Agriculture, Razi University, Kermanshah

Abstract

In order to investigate response of rapeseed genotypes (Brassica napus L.) to drought stress, an experiment was carried out with 16 genotypes and 5 level of drought induced via polyethylene glycol 6000 (0, 10, 20, 30 and 40% corresponding to osmotic potentials of control, -0.15, -0.49, -1.03 and -1.76 MPa) as a factorial arrangement in CRD template with three replications. Hypocotyl calli derived from cultures on callus induction medium (MS medium supplement with 0.5 mg/l NAA + 0.5 mg/l 2,4-D and 0.5 mg/l BAP) were subjected to different levels of drought stress. Some traits such as relative growth rate (RGR), relative water content (RWC), INTOL index, callus growth rate (CGR) and proline content (PC) were measured in calli. The results of ANOVA in vitro showed that there was a significantly difference between all genotypes for the traits. Results of means comparison revealed that the genotype No. 7 (Dante) had the highest amounts of RWC, CGR, RGR and INTOL, therefore this  genotype is introduced because it had drought tolerant calli.

Keywords

Volume 2, Issue 2 - Serial Number 2
December 2011
Pages 49-57

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