In Silico Analysis of the WRKY Transcription Factors Gene Family in Wheat

Document Type : research

Authors

1 M.Sc. Graduate of Agricultural Biotechnology, Faculty of Agriculture, Shahed University, Tehran

2 Assistant Professor of Molecular Genetics and Genetic Engineering, Agricultural Biotechnology Research Institute of Iran, Karaj

3 Assistant Professor of Plant Breeding- Biometry, Department of Biotechnology, Faculty of Agriculture, Shahed University, Tehran

Abstract

WRKY gene family encodes a large group of transcription factors which are involved in the regulation of biotic and abiotic stress responsive genes. To identify WRKY family members in wheat, multiple searches were done in the related databases. Rice WRKY conserved sequences were used as the templates for tblastn searches in the nr, EST, HTGS datasets for finding new members in wheat. EST and cDNA sequences were translated by the DNAstar software and NCBI ORF finder. Phylogenetic analyses were done using MEGA4 and BioEdit softwares. Expression pattern of this gene family studied by analysis of microarray data from the PLEXdb, GENEVESTIGATOR databases. According to the results, 94 members were found in the wheat WRKY gene family which 76 members of them had complete conserved domain. Wheat WRKY transcription factors such as rice WRKY TFs were divided into three groups based on the WRKY conserved domain. 7 members of this family with differential expression at drought stress condition in sensitive and tolerant varieties were regarded as candidate genes for drought tolerance.

Keywords

References

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Volume 6, Issue 2
May 2016
Pages 39-44

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