Study of Callus Induction of Medicinal Chavil Plant (Ferulago angulata B.) Using Types of Explants and Growth Regulators

Document Type : Research Paper

Authors

1 Former Graduate Student of Plant Breeding, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Yasouj, Yasouj

2 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Yasouj, Yasouj

3 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Yasouj, Yasouj

Abstract

Medicinalchavil plant (Ferulago angulata B.) is an important plant belonging to the Apiaceae family. In order to determination of the best explants and hormonal combination for callus induction, this experiment carried out in Yasouj University usingtypes of explants including root, shoot, leaf (separated from seedling obtained from embryo culture) and fragmented embryo and hormone combinations of NAA with BAP each of at 5 levels (0, 0.5, 1, 1.5 and 2 mg/l) and 2,4-D at 5 levels (0, 1, 2, 3 and 4 mg/l) with BAP at 5 levels (0, 0.5, 1, 1.5 and 2 mg/l) at 1/4 MS medium culture. These treatments arranged as factorial experiment using randomized completely design with three replications. Results of Analysis of Variances showed that main effects, two way interactions and explant × NAA × BAP interaction were significant (p ≤0.01) for callus induction percentage, callus fresh weight, callus dry weight, callus length, callus diameter and callus volum. In other experiment also, these effects were significant forcallus induction percentage, callus fresh weight, callus dry weight, callus length, callus diameter and callus volum. The result of experiment indicated that root explant at the all concentration of NAA had positive effect on callus induction. In addition root explant at 1 mg/l 2, 4-D and 1/5 mg/l BAP was the best treatment for callus induction. In general, because of good responses of chavil plant to callus induction, these results could be used in breeding programs of chavil plant.

Keywords

Main Subjects

References

Abrazeh, M. 2003. Investigation of some characteristics of Ferulago angulata in Dena protected area. M.Sc. Thesis of Range Management, Department of Natural Resources, Tarbiat Modarres University, 72 pp.
Arzani, A. and Mirodjagh, S. S. 1999. Response of durum wheat cultivars to immature embryo culture, callus induction and in vitro salt stress. Plant Cell, Tissue and Organ Culture, 58 (1): 67-76.
Christou, P., Ford, T. L. and Kofron, M. 1991. Production of transgenic rice (Oryza sativa L.) from organomically important indica and japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryo. Biotechnology, 9: 957-962.
Cosgrove, N. 2001. Wall structure and wall loosening. A look backwards and forwards. Plant Physiology, 125: 131-134.
Dixon, R. A. and Gonzales, R. A. 1996. Plant Cell Culture: A Practical Approach. 2nd Ed. IRL Press, 230 pp.
Ebrahimie, E., Habashi, A. A., Ghareyazie, B., Ghannadha, M. and Mohammadi, M. 2003. A rapid and efficient method for regeneration of plantlets from embryo explants of cumin (Cuminum cyminum). Plant Cell, Tissue and Organ Culture, 75: 19-25.
Farsi, M. and Zolala, J. 2003. Principles of Plant Biotechnology (translate). Ferdowsi University of Mashhad, 495 pp.
Gasper, T., Penel, C., Thorpe, T. and Greppin, H. 1982. Peroxidases: A survey of their biochemical and physiological roles in higher plants. University of Geneva press, Geneva, Switzerland. 324 pp.
Hejabi, H., Razavi, S. M., Ghasemian, A. and Vatanpour, Z. 2009. Induction callus in medicinal plant parsley (Petroselinum sativum). 12th Iranian Genetics Congress, pp 1-5.
Johri, M. M. and Mitra, D. 2001. Action of plant hormones. Current Science, 80 (2): 199-205.
Khawar, K., Sarıhan, M. E., Sevimay, C., Parmaksız, S., Uranbey, I., Ipek, S., Kaya, A., Sancak, M. D. and Ozcan, C. 2005. Adventitious shoot regeneration and micropropagation of Plantago lanceolata L., Period Biology, 107: 113-116.
Mockeviciute, R. and Anisimoviene, N. 1999. Indole-3-acetic acid receptors in the cytosol. Biology Ogija, 4: 90-93.
Nikam, T. D. and Shitole, M. G. 1999. In vitro culture of Safflower L. cv. Bhima: initiation, growth optimization and organogenesis. Plant Cell, Tissue and Organ Culture, 5: 15-22.
Nori-Ghanballani, G. 1992. Experiences and background in plant tissue culture. Tabriz University Press, 411 pp.
Omidi, M. and Farzin, N. 2009. Biotechnology approaches for improvement of medicinal plants. Food and Biotechnology Congress, Kermanshah Azad University, pp 1-14.
Safarnejad, A. 2011. A Rapid and efficient method for regeneration of Cuminum setifolium (Boiss.). International Journal of Science and Nature, 2 (4): 737-742.
Sarabadani Tafreshi, R., Omidi, M., Bihamta, M. R. and Davazdahemami, S. 2008. Study of in vitro embryo culture and Effect of explants, different hormonal levels on callus induction and shooting of Ferula gummosa. Journal of Medicinal Plants, 27: 71-81.
Sarkheil, P., Omidi, M., Peyghambari, S. A. and Davazdahemami, S. 2009. The effects of plant growth regulators and explants on callogenesis regeneration and suspension culture in Foeniculum vulgare Mill. Iranian Journal of Medicinal and Aromatic Plants, 25 (3): 364-375.
Schultz, W. S., Hose, R., AbonMandou, A. and Czygan, F. C. 1990. Melissa officinalis L. (Lemon balm), In vitro culture and the production and analysis of volatile compounds, Biotechnology in Agriculture and Forestry, 24: 242.
Shahadati-Moghadam, Z. 2001. Plant growth regulators. M.Sc. Thesis of Agriculture, Mazandaran University, Iran. 125 pp.
Sharifi, M. 1995. Comparitive investigation of essences of Bunium persicum and Cuminum cyminum seed and explant fragments. M.Sc. Thesis, Faculty of Agriculture, Tehran University, Iran.144 pp.
Shishova, M. F., Lindberg, S. and Polevoi, V. V. 1999. Auxin activation of Ca2+ transport across the plasmalemma of plant cells. Russian Journal of Plant Physiology, 46 (5): 626-633.
Soltanipoul, M., Mohammadi, E., Rahnama, H. and Abbaszadeh, B. 2011. Callusogenesis investigation of Lemon balm (Melissa officinalis L.). Journal of Agronomy and Plant Breeding, 7 (1): 45-54.
Theiler, R. and Kagi, A. C. 1991. Cloning in vitro and somatic embryogenesis in Foeniculum vulgare Mill. (fennel) of Zeta fino and Zefa tard. Acta Horticulturae, 300: 287-291.
Valizadeh, M., Safarnejad, A., Nematzadeh, G. A. and Kazemitabar, S. K. 2006. Regeneration of plantlets from embryo explants of Bunium persicum (Boiss). Indian Journal Crop Science, 1 (1-2): 93-96.
Valizadeh, M., Safarnejad, A., Nematzadeh, G. H., Kazemitabar, K. and Hamidi, H. 2008. Regeneration of plant lets from fragmented embryo explant of Parsi Zira (Bunium persicum Boiss.). Journal of Seedling and Seed, 24 (3): 389-398.
Zare, A., Solouki, R., Omidi, M., Irvani, M., Mahdi Nezad, N. and Rezazadeh, S. H. 2010. Callus induction and plant regeneration in Ferula assa-foetida L. (Asafetida), an endangered medicinal plant. Trakia Journal of Sciences, 8 (1): 11-18.
Zhang, L. and Lu, Y. T. 2003. Calmodulin-binding protein kinases in plants. Trends Plant Science, 8: 123-127.
Volume 6, Issue 2
May 2016
Pages 73-80

Files

Share

How to cite

Statistics