Investigation of Salinity Effect on Leaf Proteome Pattern of Triticum boeoticum

Document Type : research

Authors

1 M.Sc. Student of Plant Breeding, Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman

2 Assistant Professor, Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman

3 Associate Professor of Plant Breeding, Faculty of Agriculture, Shahid Bahonar University, Kerman

4 Associate Professor, Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman

5 Assistant professor of Biochemistry, Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman

6 M.Sc. of Plant Breeding, Department of Plant Breeding, Faculty of Agriculture,Tehran University, Karaj

Abstract

Wild relatives of crop plants is an important resource for finding the genes conferring tolerance to salinity. In this study, ten different ecotypes of Triticum boeoticum were planted in greenhouse by using factorial experiment based on completely randomized design. Salt stress was applied at the three-leaf stage and at two levels: zero and 150 mM and were sampled after 15 days from newly full developed blade leaf. The concentration of sodium and potassium were measured in each sample and after that the tolerant ecotype was determined based on concentration of Na+ and K+/Na+ trait. Leaf sample of tolerant ecotype were used for two dimensional electrophoresis. Extracted proteins first isolated based on isoelectric point by using IPG strip with pH 3-10 and then isolated based on molecular weight in the second dimension. The result showed that C11 and C7 that collected from Saghez and Kamiaran respectively were most tolerant populations. Both of B2 and C1 that collected from Harsin and Sanandaj respectively were most sensitive populations. Based on physiological traits, sample C11 was selected for proteomics. According to the proteomics results, the number of repetitive 177 gel spots were identified and statistically analyzed. From total spots, 13 spots showed differential expression under salt stress, from these, the expression of 8 spots (61.5%) increased and 5 spots (38.5%) decreased under salt stress. This means that the C11 is trying to cope with salinity stress by changing the expression of their responsive genes. 

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References

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Volume 7, Issue 1
June 2016
Pages 61-69

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