Evaluation of Genetic Diversity in Sisymbrium irio Germplasm Using ISSR Marker

Document Type : research

Authors

1 Assistant Professor, Department Biotechnology, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan

2 M.Sc. Student, Department Biotechnology, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan

Abstract

Sisymbrium irio or bitter flixweed is important among the medicinal plants. The human population overgrowth and absence of a comprehensive management of natural resources caused a fast decrease in most valuable indigenous germplasm. Management of genetic diversity of native plants is important in conservation programs. Using molecular techniques, especially ISSR, one can study genetic diversity and analyze the genetic variation. In this study the genetic diversity of 33 populations of S. irio has been investigated by Inter Simple Sequence Repeat (ISSR) marker. In total, 183 sites were investigated. 11 ISSR primers in this study showed 100% polymorphism. The average polymorphism information content (PIC) was equal to 31.5. The minimum amount of PIC for primer (AG) 8T was equal to 24.5 and maximum amount of PIC for primer GGGC (GA) 8 was equal to 038/5. The maximum amount of MI for primer (AG) 8YT was equal to 21.82, therefore it is the most proper primer for further studies. Results showed high variation among and between genotypes and there is the chance of selection and modification.

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Main Subjects

References

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Volume 7, Issue 1
June 2016
Pages 71-78

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