Study of Endosperm Protein Banding Pattern Variation in some of Wheat (Triticum aestivum L.) Genotypes by SDS-PAGE Method

Document Type : research

Authors

1 Assistant, Department of Agriculture, Payame Noor University, 19395-4697, I. R. of IRAN

2 Instructor, Department of Agriculture, Payame Noor University, 19395-4697, I. R. of IRAN

3 M.Sc. Graduated, Department of Plant Protection, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan

Abstract

Knowledge of genetic diversity in breeding programs based on various marker, including parental choice is important. The most important part of plant breeding programs understanding of genetic diversity to classify population based on different markers for selecting the suitable parents. In this research, genetic diversity of 16 genotypes of wheat was evaluated using SDS-PAGE protein markers. Extraction of endosperm proteins and then concentration of proteins was measured by Bradford method. For separating and patterning of the extracted proteins, SDS-PAGE technique via poly acrylamide electrophoresis was used. After staining of proteins by coomassie blue R-250 and scoring of the bands, cluster analysis was computed based on Jaccard coefficient which that the genotypes classified into 3 separate groups. Thus according to genetic distances between the genotypes, we can use cross between Omid and Gaspard for obtain the highest amount of heterosis in future breeding program.

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References

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Volume 7, Issue 2
March 2017
Pages 77-83

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