Identification of Fusarium Species Isolated from Crude Oil Contaminated Soils in Oil Refinery in Kermanshah Province

Document Type : research

Authors

1 Assistant Professor, Department of Biology, Faculty of Sciences, Razi University, Kermanshah

2 MSc Student of Microbiology, Science and Research Branch, Islamic Azad University, Kurdistan, Sanandaj, Iran

3 Associate Professor, Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan

Abstract

Members of Fusarium species are common Fungi that can metabolize of petroleum hydrocarbons in entire the world. These fungi are resistant in oil contaminated sites.Therefore, the objectives in this study were to isolate and identify of Fusarium spp. from oil-contaminated soils in oil refinery in Kermanshah province based on the morphological and molecular characteristics. Crude oil contaminated soil samples were collected from crude oil contaminated soils in oil refinery in Kermanshah province. Fusarium spp. was isolated using peptone  pentachloronitrobenzene agar (PPA) plates  supplemented with streptomycin and neomycin. In this research 30 Fusarium strains were isolated. Based on morphological characters, all isolates belonged to F. falciforme, F. keratoplasticum, and F. petroliphilum which belonged to Fusarium solani species complex. Based on the sequence data from ITS rDNA analysis, Fusarium isolates were divided into three major groups. Although the data obtained from morphological and molecular studies sufficiently supported each other, the resulting phylogenetic trees based on ITS rDNA dataset clearly distinguished closely related species and distinctly separated all morphological taxa. All the Fusarium spp. related from oil-contaminated soils was reported for the first time in Iran. Existing of fungi particularly Fusarium species in crude oil soils shows the probability of degradation and consumption of oil contaminated by these fungi. 

Keywords

Main Subjects

References

Al-Nasrawi, H. A. 2012. Biodegradation of crude oil by fungi isolated from Gulf of Mexico. Journal of Bioremediation and Biodegradation, 3(4): 147-52.
April, T. M., Fought, J. M. and Currah, R. S. 2000. Hydrocarbon-degrading filamentous fungi isolated from flare pit soils in northern and western Canada. Canadian Journal of Microbiology, 46(1): 38-49.
Burgess, L. W. 1981. General ecology of the fusaria, p. 225-235. In Nelson, P. E., Toussoun, T. A. and Cook, R. J. (ed.). Fusarium: diseases, biology, and taxonomy. Pennsylvania State University Press, University Park.
Chaillan, F., Fleche, A. L., Bury, E., Phantavong, Y., Crimount, P. and Saliot, A. 2004. Identification and biodegradation potential of tropical aerobic hydrocarbon-degradading microorganisms. Research in Microbiology, 155(7): 587-95.
Davari, M. and Arzanlou, M. 2011. Identification of some fungal species involved in biodegradation of petroleum pollutants in Northwest of Iran. Rostaniha, 12: 1-12.
Dawoodi, V., Madani, M. and Tahmourespour, A. 2014. Flora of soil fungi in Khuzestan province's oil regions. Biological Journal of Microorganism, 10: 87-96.
Gadd, G. M., Watkinson, S. C. and Dyer, P. S. 2007. Fungi in the environment, New York: Cambridge University.
Lemos, J. L. S., Rizzo, A. C., Millioli, V. S., Soriano, A. U., Sarquis, M. I. and Santos, S. 2002. Petroleum degradation by filamentous fungi. Proceeding of 9th Annual International Petroleum Environmental Conference; Oct 21-25, Albuquerque NM, EUA. P: 738- 47.
Leslie, J. F. and Summerell, B. A. 2006. The Fusarium Laboratory Manual. UK: Blackwell Publishing Ltd. 388pp.
Murray, M. G. and Thompson, W. F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acid Research, 8: 4321-4325.
Nalim, F. A., Samuels, G. J., Wijesundera, R. L. and Geiser, D. M. 2011. New species from the Fusarium solani species complex derived from perithecia and soil in the Old World tropics. Mycologia, 103: 1302-1330.
Nash, S. M., Snyder, W. C. 1962. Quantitative estimation by plate counts of propagules of the bean root rot Fusarium in field soils. Phytopathology, 52: 567-572.
Obire, O., Anyanwu, E. C. and Okigbo, R. N. 2008. Saprophytic and crude oil-degrading fungi from cow dung and poultry droppings as bioremediating agents. Journal of Agricultural Science and Technology, 4(2): 81-89.
Obire, O. and Anyanwu, E. C. 2009. Impact of various concentrations of crude oil on fungal populations of soil. International Journal of Environmental Science and Technology, 6(2): 211-218.
Onifade, A. K. and Abubakar, F. A. 2007. Characterization of hydrocarbon-degrading microorganisms isolated from crude oil contaminated soil and remediation of the soil by enhanced natural attenuation. Research in Microbiology, 2(2): 149-55.
Short, D. P. G., O’Donnell, K., Thrane, U., Nielsen, K. F., Zhang, N., Juba, J. H. and Geiser, D. M. 2013. Phylogenetic relationships among members of the Fusarium solani species complex in human infections and the descriptions of F. keratoplasticum sp. nov. and F. petroliphilum stat. nov. Fungal Genetic and Biology, 53: 59-70.
Summerbell, R. and Schroers, H. J. 2002. Analysis of phylogenetic relationship of Cylindrocarpon lichenicola and Acremonium falciforme to the Fusarium solani species complex and a review of similarities in the spectrum of opportunistic infections caused by these fungi. Journal of Clininical Microbiology, 40: 2866-2875.
Thapa, B., Kumar, K. C. A. and Ghimire, A. A. 2012. Review on bioremediation of petroleum hydrocarbon contaminants in soil. Journal of Engineering Science and Technology, 8(1): 164-170.
White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M. A., Gelfand, D. H., Sninsky, J. J. and White, T. J. (Ed.) PCR protocols: A guide to methods and applications. San Diego: Academic Press, pp: 315-322.
 
Volume 8, Issue 1
June 2017
Pages 67-74

Files

Share

How to cite

Statistics