Evaluation Diversity of some Center and South Iranian Onion (Allium cepa L.) Genotypes for Bolting Resistance and It`s Relationship with SSR Marker

Document Type : research

Authors

1 Assistant Professor, Department of Plant Breeding, Faculty of Agriculture, Yasouj University, Yasouj

2 MSc Student, Department of Plant Breeding, Faculty of Agriculture, Yasouj University, Yasouj

3 Associate Professor, Department of Plant Breeding, Faculty of Agriculture, Yasouj University, Yasouj

Abstract

Flowering branches formation in bulb production time were reduced the quality and quantity of product. Thus, study how this phenomenon for identifying bolting resistant genotypes is important. To evaluate diversity of some center and south Iranian onion genotypes in respect to flower production, 16 accessions of Iranian onions seeds (6 genotype received from Iranian Gene bank and 10 local accessions were collected from different regions of the center and south of the country) as well as two foreign cultivars were planted in the pots and in stage of eight leaves, were treated during 45 and 70 day. From 12 SSR primers, only 10 primers can produced right bands. The average polymorphic Information Content (PIC), observed and expected heterozygosity were 0.38, 0.49 and 0.59, respectively. Cluster analysis based on molecular data using Jaccard's similarity coefficient and UPGMA method, classified the evaluated genotypes in six groups. The results of binary logistic regression showed that some of SSR markers are linked to morphological and physiological characteristics.

Keywords

Main Subjects


Abdollahi-Sisi, N., Mohammadi, S. M., Alavi-kia, S. S. and Sadeghzadeh, B. 2012. Efficiency of EST-SSR markers in determination genetic diversity and relationships of barley landraces. Cereal Research, 93:123-133.
Ahmadi Mashgeneni, F., Nasr Esfahani, M. and Ebrahimi, M. A. 2014. Genetic diversity of Iranian onion genotypes using RAPD marker. Advanced Genetic Journal, 10(2): 299-303. 
Alemzadeh-Ansari, N. 2009. Onion. First edition. Shahid Chamran University Publication. 238pp.
Anderson, B. and McDonald, G. 1993. Construction of DNA libraries of A-T rich organisms using EcoRI star activity. Annual Review of Biochemistry, 211: 325-327.
Brewster, J. L. 1982. Flowering and seed production in overwintered cultivars of bulb onion. II. Quantitative relationships between mean temperatures and day lengths and the rate of inflorescence development. Journal of Horticultural Science, 57: 103-108.
Brewster, J. L. 1985. The influence of seedling size and carbohydrate status and of photon flux density during vernalization on inflorescence initiation in onion (Allium cepa L.). Annuals of Botany, 55: 403-414.
Chen, Z. W., Lu, R. J., Zou, L., Du, Z. Z., Gao, R. H., He, T. and Huang, J. H. 2012. Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China. Genetic and Molecular Research, 11: 644-650.
Dannequin, L. T. M., Panaud, O., Robert, T. and Ricroch, A. 1997. Assessment of genetic relationship among sexual and asexual forms of Allium cepa using morphological traits and RAPD markers. Heredity Journal, 78: 403-409.
Falconer, D. S. 1989. Introduction to Quantitative Genetics. (3rd edition), Longman, New York. 415 pp.
Fischer, D. and Bachman, K. 2000. Onion Microsatellites for germplasm analysis and their use in assessing intera and inter specific relatedness within the subgenus rhizirideum. Theoretical and Applied Genetics, 101: 153-164.
Kara, M. and Mishra, D. 1976. Catalase, Peroxidase and polyphenolxidase activities during rice leaf senescence. Plant Physiology Journal, 57: 315-319.
Karimi Nafchi, Z., Seyed Tabatabaei, E. and Mobli, M. 2011. Genetic diversity of onion genotypes using microsatellite markers. Iranian Journal of Horticultural Science, 42(1): 11-20.
Khokhar, K. M., Hadley, P. and Pearson, S. 2007. Effect of cold temperature duration of onion sets in store on the incidence of bolting, bulbing and seed yield. Scientia Horticulturae, 112: 16-22.
Mahajan V. Akse, J. Havey, M. J. and Lawande, K. E. 2009. Genetic fingerprinting of onion cultivars using SSR mark­ers. Indian Journal of Horticulture, 66: 62-68.
McCallum, J., Thomson, S. and Kenel, F. 2006. Assessment of genetic diversity in bulb onion (Allium cepa L.) using simple sequence repeat. San Digobusiness Journal, PP.130.
Moosavizadeh, A., Toorchi, M., Moghaddam, M., Mohammadi, S. A. and Masiha, S. 2005. Genetic diversity of Iranian onion landraces using RAPD marker. Agriculture Science, 16(1): 265-277.
Murray, G. C. and Thampson, W. F. 1980. Rapid isolation of high molecular DNA. Nucleic Acid Resources, 8: 4321-4325.
Pirseyedi, S. M., Mardi, M., Naghavi, M. R., Iran Doost, H. P., Sadeghzadeh, D., Mohammadi, S. A. and Ghareyazie, B. 2006. Evaluation of genetic diversity and Identification of informative markers for morphological characters in Sardari derivative wheat lines. Pakistan Journal of Biological Sciences, 9: 2411-2418.
Ribeiro-Carvalho, C., Guedes-Pinto, H. and Igregas, G. 2004. High levels of genetic diversity throughout the range of the Portuguese wheat landrace Barbela. Annals of Botany Journal, 94: 699-705.
Shourvazdi, A., Mohammadi, S. A., Norozi, M. and Sadeghzadeh, B. 2014. Molecualr analysis of genetic diversity and relationships of barley landraces based on microsatellite markers. Journal of Plant Genetic Research, 1(1): 51-64. 
Wiebe, H. J. 1990. Vernalization of vegetable crops- A review. Acta Horticulturae Journal, 267: 323-328.
Yun Hyun, D., Kim, O. Y., Bang, K. H., Kim, Y. Ch., Yoo, N. H., Kim, Ch. W. and Lee, J. H. 2009. Genetic and molecular studies for regulation of bolting time of onion (Allium cepa L.). Journal of Plant Biology, 52: 602-608.