Antioxidant Response of Mexican Lime to Xanthomonas citri subsp. citri

Document Type : research

Authors

1 PhD Student, Department of Plant Pathology, Faculty of Agriculture, Ferdowsi Uinversity of Mashhad, Mashhad

2 Associate Professor, Department of Plant Pathology, Faculty of Agriculture, Ferdowsi Uinversity of Mashhad, Mashhad

3 Associate Professor, Department of Plant Protection, Iran Citrus Research Institute, Ramsar, Iran

Abstract

Mexican lime(Citrus aurantifolia) is one of the susceptible citrus genotype to Xanthomonas citri subsp. citri (Xcc) which is grown in the south and south-west of Iran. The disease infects all aerial parts of Mexican lime and severely infected plants show defoliation, badly fruit, premature fruit drop, twig dieback and general tree decline. In this study, Mexican lime leaves were artificially inoculated with Xcc to determine the effect of Xcc infections on antioxidant enzymes activity such as superoxide dismutase (SOD), guaiacol peroxidase (GP), catalase (CAT) and ascorbate peroxidase (APOD). These results indicated that CAT activity was significantly higher in infected leaves than controls. No effect on SOD activity was observed in infected leaves. The result also showed that POD was significantly increased at 2, 5 and 7 days post inoculation. While, APOD activity was higher than control in inoculated leaves during infected period with Xcc, considering that the results was significant only in 2 and 10 days post inoculation. Current results can present new approaches in the future researches in order to control CBC via transformation of plant defense.

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References

Alizadeh, A. and Rahimian, H. 1990. Citrus canker in Kerman province. Iranian Journal of Plant Pathology, 26: 1-4.
Al-Saleh, M. A., Widyawan, A., Saleh, A. A. and Ibrahim, Y. E. 2014. Distribution and pathotype identification of Xanthomonas citri subsp. citri recovered from south-western region of Saudi Arabia. African Journal of Microbiology Research, 8(7): 673-679.
Amaral, A. M., Carvalho, S. A., Silva, L. F. C. and Machado, M. A. 2010. Reaction of genotypes of Citrus species and varieties to Xanthomonas citri subsp. citri under greenhouse conditions. Journal of Plant Pathology, 92(2): 519-524.
Barbedo, J. G. A. 2013. Digital image processing techniques for detecting, quantifying and classifying plant diseases. SpringerPlus, 2(660): 1-12.
Barna, B., Fodor, J., Pogany, M. and Kiraly, Z. 2003. Role of reactive oxygen species and antioxidants in plant disease resistance. Pest Management Science, 59: 459-464.
Bradford, M. M. 1976. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein- dye binding. Analytical Biochemistry, 72: 248-254.
Chen, P. S., Wang, L. Y., Chen, Y. J., Tzeng, K. C., Chang, S. C., Chung, K. R. and Lee, M. H. 2012. Understanding cellular defence in kumquat and calamondin to citrus canker caused by Xanthomonas citri subsp. citri. Physiological and Molecular Plant Pathology, 79: 1-12.
Choudhary, R., Saroha, A. E. and Swarnkar, P. L. 2012. Effect of abscisic acid and hydrogen peroxide on antioxidant enzymes in Syzygium cumini plant. Journal of Food Science and Technology, 49(5): 649-652.
Daneshjoo, A., Abbaspour-Fard, M. H., Aghkhani, M. H. and Arian, M. 2008. Introducing and evaluating a suitable software for sprayers calibration and other similar purposes. Journal of Plant Protection (Agricultural Science and Technology), 22(2): 171-181.
Das, A. K. 2003. Citrus canker, A Review. Journal of Applied Horticulture, 5(1): 52-60.
De Vleesschauwer, D., Chernin, L. and Höfte, M. 2009. Differential effectiveness of Serratia plymuthica IC1270-induced systemic resistance against hemibiotrophic and necrotrophic leaf pathogens in rice. BMC Plant Biology, 9(9): 1-16.
Deng, Z. N., Xu, L., Li, D. Z., Long, G. Y., Liu, L. P., Fang, F. and Shu, G. P. 2010. Screening citrus genotypes for resistance to canker disease (Xanthomonas axonopodis pv. citri). Plant Breeding, 129(3): 341-345.
Dhaygude, S. and Kumbhar, N. P. 2013. Agricultural plant leaf disease detection using image processing. International Journal of Advanced Research in Electrical, Electronics and Instrumentation Engineering, 1(2): 599-602.
Dhindsa, R. A., Plumb-Dhindsa, P. and Thorpe, T. A. 1981. Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany, 126: 93-101.
Ebel, R. C. and Kumar, N. 2012. Interference of oxidative metabolism in citrus by Xanthomonas citri pv. citri. In: Volodymyr Lushchak (Ed.). Oxidative stress environmental induction and dietary antioxidants, INTECH Open Access Publisher, Croatia, 169-188 pp.
Fahimirad, S., Karimzadeh, G. and Ghanati, F. 2013. Cold-induced changes of antioxidant enzymes activity and lipid peroxidation in two canola (Brassica napus L.) cultivars. Journal of Plant Physiology and Breeding, 3(1): 1-11.
Gottwald, T. R., Graham, J. H., Civerolo, E. L., Barrett, H. C. and Hearn, C. J. 1993. Differential host range reaction of citrus and citrus relatives to citrus canker and citrus bacterial spot determined by leaf mesophyll susceptibility. Plant Disease, 77: 1004-1009.
Hasabi, V., Askari, H., Alavi, S. M. and Zamanizadeh, H. 2014. Effect of amino acid application on induced resistance against citrus canker disease in lime plants. Journal of Plant Protection Research, 54: 144-149.
Hung, S. H., Yu, C. W. and Lin, C. H. 2005. Hydrogen peroxide functions as a stress signal in plants. Botanical Bulletin- Academia Sinica Taipei Journal, 46: 1-10.
Khalaf, A., Moore, G. A., Jones, J. B. and Gmitter, F. G. 2007. New insights into the resistance of Nagami kumquat to canker disease. Physiological and Molecular Plant Pathology, 71(4): 240-250.
Khodakaramian, G., Rahimian, H., Mohamadi, M. and Alameh, A. 1999. Phenotypic features, host range and distribution of the strains Xanthomonas axonopodis including citrus canker in southern Iran. Iranian Journal of Plant Pathology, 35: 102-111.
Kumar, N., Ebel, R. C. and Roberts, P. D. 2011a. H2O2 degradation is suppressed in kumquat leaves infected with Xanthomonas axonopodis pv. citri. Scientia Horticulturae, 130(1): 241-247.
Kumar, N., Ebel, R. C. and Roberts, P. D. 2011b. H2O2 metabolism during sweet orange (Citrus sinensis L. Osb.)‘Hamlin’ Xanthomonas axonopodis pv. citri interaction. Scientia Horticulturae, 128(4): 465-472.
Kumar, N., Ebel, R. C. and Roberts, P. D. 2011c. SOD activity in Xanthomonas axonopodis pv. citri infected leaves of kumquat. Journal of Horticultural Science and Biotechnology, 86: 62-68.
Kumar, S. and Kaur, R. 2015. Plant disease detection using image processing- a review. International Journal of Computer Applications, 124(16): 6-9.
Leduc, A., Traoré, Y. N., Boyer, K., Magne, M., Grygiel, P., Juhasz, C. C., Boyer, C., Guerin, F., Wonni, I. and Ouedraogo, L. 2015. Bridgehead invasion of a monomorphic plant pathogenic bacterium: Xanthomonas citri pv. citri, an emerging citrus pathogen in Mali and Burkina Faso. Environmental Microbiology, 17(11): 4429-4442.
Li, J. and Wang, N. 2012. The gpsX gene encoding a glycosyltransferase is important for polysaccharide production and required for full virulence in Xanthomonas citri subsp. citri. BMC Microbiology, 12(1): 1-16.
Mohammadi, M., Mirzaee, M. R. and Rahimian, H. 2001. Physiological and biochemical characteristics of Iranian strains of Xanthomonas axonopodis pv. citri, the causal agent of citrus bacterial canker disease. Journal of Phytopathology, 149(2): 65-75.
Mustafa, M., Imran, M., Rasool, A., Azeem, M., Riaz, A. and Afzal, M. 2014. Evaluation of commercial citrus cultivars for resistance to citrus leaf miner and its management. International Journal of Agronomy and Agricultural Research, 6(6): 1-9.
Park, D. S., Hyun, J. W., Park, Y. J., Kim, J. S., Kang, H. W., Hahn, J. H. and Go, S. J. 2006. Sensitive and specific detection of Xanthomonas axonopodis pv. citri by PCR using pathovar specific primers based on hrpW gene sequences. Microbiological Research, 161(2): 145-149.
Pérez‐Clemente, R. M., Montoliu, A., Vives, V., López‐Climent, M. F. and Gómez‐Cadenas, A. 2015. Photosynthetic and antioxidant responses of Mexican lime (Citrus aurantifolia) plants to Citrus tristeza virus infection. Plant Pathology, 64(1): 16-24.
Pruvost, O., Goodarzi, T., Boyer, K., Soltaninejad, H., Escalon, A., Alavi, S. M., Javegny, S., Boyer, C., Cottyn, B. and Gagnevin, L. 2015. Genetic structure analysis of strains causing citrus canker in Iran reveals the presence of two different lineages of Xanthomonas citri pv. citri pathotype A. Plant Pathology, 64(4): 776-784.
Rasoulnia, A., Alavi, S. M., Askari, H., Farrokhi, N. and Soltani Najafabadi, M. 2013. Antioxidant activity and lipid peroxidation in response to citrus canker bacterial infection. International Journal of Farming and Allied Sciences, 2(24): 1179-1184.
Rezaei, M., Shams-Bakhsh, M. and Alizadeh, A. 2012. Genetic diversity among Xanthomonas citri subsp. citri strains in Iran. Journal of Plant Protection Research, 52(1): 1-9.
Schaad, N. W., Jones, J. B. and Chum, W. 2001. Laboratory Guide for Identification of Plant Pathogenic Bacteria. 3rd Ed. APS Press, St. Paul, MN, USA. 175-193 pp.
Taheri, P., Irannejad, A., Goldani, M. and Tarighi, S. 2014. Oxidative burst and enzymatic antioxidant systems in rice plants during interaction with Alternaria alternate. European Journal of Plant Pathology, 140: 829-839.
Taheri, P. and Tarighi, S. 2009. Riboflavin induces resistance in rice against Rhizoctonia solani via jasmonate-mediated priming of phenylpropanoid pathway. Journal of Plant Physiology, 167: 201-208.
Tertivanidis, K., Doudoula, C., Vasilikiotis, C., Hassiotou, E., Perl-Treves, P. and Tsaftaris, A. 2004. Superoxide dismutase transgenes in sugarbeets confer resistance to oxidative agents and the fungus C. beticola. Transgenic Research, 13(3): 225-233.
Voludakis, A. E., Marmey, P., Delannoy, E., Jalloul, A., Martinez, C. and Nicole, M. 2006. Molecular cloning and characterization of Gossypium hirsutum superoxide dismutase gene during cotton-Xanthomonas campestris pv. malvacerum interaction. Physiological and Molecular Plant Pathology, 68: 119-127.
Wang, Y., Fu, X. Z., Liu, J. H. and Hong, N. 2011. Differential structure and physiological response to canker challenge between Meiwa kumquat and ‘Newhall’ navel orange with contrasting resistance. Scientia Horticulturae, 128(2): 115-123.
Volume 8, Issue 2
September 2017
Pages 9-17

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