Genetic Evaluation of Cumin and Caraway Using Eryngium planum Microsatellite Markers

Document Type : research

Authors

1 MSc Student, Department of Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol

2 Assistant Professor, Department of Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol

3 Lecturer Research, Department of Agronomy and Plant Breeding, Agricultural Research Institute, University of Zabol, Zabol

Abstract

Medicinal plants have special importance in terms of public health promotion and disease prevention with treatment. Among these medicinal plants, black and green cumin have a special place. The aim of this study was to evaluate the genetic similarity and genetic distance between two group (10 population) of cumin and caraway based on SSR markers. In Cumin, the number of allele amplified per primer was different from 2 (Ealp024) to 5 (Ealp245 and Ealp741) with average 3.25 and also in caraway, was from 2 (Ealp017, Ealp741, Ealp1349 and EalpD268) from 6 (Ealp1479 and Ealp024) with average (3.5). In Cumin, diversity index was different from 0.48 (Ealp024) to 0.89 (Ealp017) with average 0.61 and also in caraway was from 0.32 (Ealp741) to 0.8 (Ealp1479) with average (0.61). The highest Shanon diversity (0.28) and Nei diversity (0.20) were belong to caraway population of Neishabor and Jandagh and the lowest Shanon diversity (0.057 and 0.056) and Nei diversity (0.04) were belong to cumin population of Sabzvar and Neishabor. The fartherest population among cumin were cumin of Sabzvar and Gazbarkhoar and among caraway were caraway of Kerman and Neishabor. Partitioning variations within and between populations, using an analysis of molecular variance (AMOVA), showed that 13% and 87% of the total genetic variation respectively existed between and within growing regions. Eryngium planum Microsatellite Markers that were used and amplified in Cumin and Caraway approved evolution process and the present conserve sequence between Cumin, Caraway and Eryngium planum.

Keywords

Main Subjects

References

Agrama, H. A. and Tuinstra, M. R. 2003. Phylogenetic diversity and relationship among sorghum accessions using SSRs and RAPDs. African Journal of Biotechnology, 2(10): 334-340.
Akkaya, M. S., Bhagwat, A. A. and Cregan, P. B. 1992. Length polymorphism of simple sequence repeats of DNA in soybean. Genetics, 132: 1131-1139.
Alikhani, L., Rahmani, M. S., Shabanian, N. and Badakhshan, H. 2014. Genetic diversity assessment of Quercus infectoria and Q. libani populations in North-Zagros forests based on ISSR and IRAP markers. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 22(1): 79-90
Azimzadeh, M., Amir, R., Osare, M. H., Bihamta, M. R. and Frotan, M. 2014. Evaluation of genetic diversity of Iranian Cumin (Bunium persicum Boiss) of nuclear ribosomal DNA using ITS. The Journal of Genetic Research and Plant Breeding, 22(1): 1-10.
Bahari, Z., Shojaeiyan, A., Rashidi Monfared, S., Mirshekari, A., Nasiri, K. and Amiriyan, M. 2015. Investigation of genetic diversity among some Iranian Dill (Anethum graveolens L.) landraces, using ISSR markers. Journal of Plant Genetic Research, 2(1):11-22.
Bahraminejad, A., Mohammadi-Nejad, G., Kadir, M. A. and Yusop, M. R. B. 2012. Molecular diversity of Cumin (Cuminum cyminum L.) using RAPD markers. Australian Journal of Crop Science, 6(2): 194-199.
Bohn, M., Friedrich, H. and Melchinger, A. E. 1999. Genetic similarities among winter wheat cultivars determined on the basis of RFLPs, AFLPs, and SSRs and their use for predicting progeny variance. Crop Science, 39: 228-237.
Botstein, D., White, R. L., Skolnick, M. and Davis, R. W. 1980. Constraction of genetic linkage map in man using restriction fragment length polymorphism. American Journal of Human Genetics, 32: 314-331.
Bryan, G. J., Collins, A. J., Stephanson, P., Orry, A., Smith, J. B. and Gale, M. D. 1997. Isolation and characterisation of microsatellite from hexaploid bread wheat. Theoretical and Applied Genetics, 94: 557-563.
Dehghan-kohestani, S., Amin-Baghizadeh, A., Ranjbar, G. A. and Babaeian-Jelodar, N. 2008. Genetic diversity of germplasm cumin [Bunium persicum (Boiss.) B. Fedtsch.] Kerman province using molecular markers RAPD. Quarterly Scientific-Research Medicinal and Aromatic Plants of Iran, 24(4): 427-414.
Dellaporta, S. L., Wood, J. and Hicks, J. B. 1993. A plant DNA minipreparation: version II. Plant Molecular Biology Reporter, 1: 19-22.
Drinic, M. S., Trifunovic, S., Drinic, G. and Konstantinov, K. 2002. Genetic divergence and its correlation to heterosis in maize as revealed by SSR-based markers. Maydica, 47: 1-8.
Fahima, T., Roder, M., Grama, A. and Nevo, E. 1998. Microsatellite DNA polymorphism divergence in Triticum dicoccoides accessions highly resistant to yellow rust. Theoretical and Applied Genetics, 96: 187-195.
Fazeli-Nasab, B. and Naghavi, M. R. 2011. Identification of Rust resistance among wheat cultiras by using SSRs markers. Journal of Agronomy Science, 4(5): 81-88.
Fazeli-Nasab, B., Mehrabi, A. A. and Izadi-Darbandi, A. 2010. Genetic diversity of wheat storage proteins and SSRs markers. Modern Genetics, 5(2): 81-93.
Fazeli-Nasab, B., Naghavi, M. R., Mardi, M., Yazdi-Samadi, B. and Kazemi, M. 2006. Genetic diversity and relationships of Iranian wheat cultivars using microsatellite markers. Iranian Journal of Agriculture Science, 37(1): 93-99.
FeiZho, H., Zhong-Wen, X. and Song, G. 2003. Microsatellite analysis of genetic diversity and population genetic structure of wild rice(Oryza rufipogen griff)in China. Theoretical and Applied Genetics, 107: 332-339.
Ganj-Khanloo, A., Mohamadi, S. A., Ghasemi-Glaazani, K., Shakiba, M. R. and Yusefi, A. 2012. Genetic diversity in barley as revealed by microsatellite markers and association analysis of these markers by traits related to freezing tolerance. Seed and Plant Improvement Journal, 1-28(1): 101-114.
Gaudeul, M., Naciri, Y., Guathier, P. and Pompanon, F. 2002. Isolation and characterization of microsatellites in a perennial Apiaceae, Eryngium alpinum L., Molecular Ecology, 2: 107-109.
Gupta, P. K., Balyan, H. S., Edwards, K. J., Isaac, P., Korzun, V., Roder, M., Gautier, M. F., Schlatter, A. R., Dubcovsky, J., Delapena, R. C., Khairallah, M., Penner, G., Hayden, M. G., Sharp, P., Keller, B., Wang, R. C. C., Hardouin, J. P., Jack, P. and Leroy, P. 2002. Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theoretical and Applied Genetics, 105: 413-422.
Haghir-alsadat, B. F., Bernard, F., Kalantar, S., Sheikhha, M. H., Homellahi, F., Azimzadeh, M. and Hori, M. 2010. Evaluation of effective compounds and antioxidant properties of essential oil of caraway of Yazd province. Yazd University of Medical Sciences Journal, 18(4): 284-291.
Halton, T. A. 2001. Plant Genotyping by Analysis of Microsatellite. In Henry, R. J. (Ed). Plant Genotyping, The DNA Fingerprinting of Plant. pp: 15-29.
Hamrick, J. L. and Godt, M. J. W. 1996. Effects of life history traits on genetic diversity in plant species. Philosophical Transaction of the Royal Society of London B Series: Biological Science, 351: 1292-1298.
Hashemi, H., Safarnejad, A. and Baghery, A. 2010. The use of RAPD marker assessing the genetic diversity of Bunium persicum B. Fedtsch populations. International Journal of Science and Nature, 1: 202-208.
Hokanson, S. C., Szewc-McFadden, A. K., Lamboy, W. F. and McFerson, J. R. 1998. Microsatellite (SSR) markers reveal genetic identities, genetic diversity and relationships in a Malus×domestica borkh core subset collection. Theoretical and Applied Genetics, 97: 671-683.
Jaccard, P. 1912. The distribution of flora in the alpine zone. The New Phytologist, 11(2): 37-50.
Jahansooz, F., Najafi, A. A., Sefidkon, F., Ebrahimzadeh, H. and Mardi, M. 2008. Assessment of genetic diversity in ecotypes of Bunium persicum using AFLP markers. The 5th national biotechnology congress of Iran, Tehran, Iran. pp. 199.
Jamshidi-Jam, F., Rabiei, V., Fotovat, R. and Taheri, M. 2014. Genetic diversity among olive clones of Zard and Rowghani cultivars using microsatellite markers. Seed and Plant Improvment Journal, 30(2): 327-345.
Kafi, M., Rashed Mohasel, M. H., Kochaki, A. and Mplafiaei, A. 2002. Cumin, produced and production technology. Ferdowsi University of Mashhad Institute Press, 200 p.
Kameli, M., Hesamzadeh, H.S.M. and Ebadi. M. 2013. Assessment of genetic diversity on populations of three satureja species in Iran using PCR markers. Annals of Biological Research, 4: 64-72.
Karimi-Afshar, A., Baghizadeh, A., Mohammadi-Nejad, G. and Abedi, J. 2014. Evaluation of genetic variation and relationships between traits in cumin (Cuminum cyminum L.) based on morphological characteristic. 1th International and 13th National Iranian Genetic Congress.
Lewontin, R. C. 1972. The apportionment of human diversity. Evolutionary Biology Journal, 6(38): 381-398.
Ma, Z. Q., Roder, M. and Sorrelis, M. E. 1996. Frequencies and sequence characteristics of di-, tri-, and tetra-nucleotide microsatellites in wheat. Genome, 39: 123-130.
Majeed, S. and Sharma, D. 2006. Assessment of genetic divergence of Kala Zira of different geographical races from India. Proceedings of 1st Int. Conference on Biotechnological Approaches for Alleviating Malnutrition and Human Health, Bangalore, India, p. 191.
Mohammadi, S. A. and Prassana, B. M. 2003. Analysis of genetic diversity in crop plants-salient statistical tools and considerations. Crop Science, 43: 1235-1248.
Momeni, H., Shiran, B., Khodambashi, M. and Cheghamirzaei, K. 2013. Studying of genetic diversity in some population of Fritillaria imperialis L. in Iranian Zagros region using ISSR marker and morphological traits. Iranian Journal of Horticulture, 44(1): 61-72.
Nie, M. 1973. Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of USA, 70(12): 3321-3323
Olfati, J. A., Samizade, H., Peyvast, G. A., Rabiei, B. and Khodaparast, S. A. 2012. Rleationship between genetic distance and heterosis in cucumber. International Journal of plant breeding, 6: 21-26.
Omidbegi, R. 2007. Production and processing of medicinal plants. Tarrahan Nashe Press, Tehran, 176 p.
Parashar, M., Jakhar, M. L. and Malik, C. P. 2014. A Review on biotechnology, genetic diversity in Cumin (cuminum cyminum). International Journal of Life Science and Pharma Research, 4(4): 17-34.
Pejmanmehr, M., Hasani, M. A., Fakhr-Tabatabai, S. M. and Hadian, J. 2009. Genetic diversity and segregating of populations Zyrh English (Bunium persicum (Boiss) using molecular markers RAPD. Journal of Environmental Sciences, 7(2): 76-63.
Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S. and Rafalsky, A. 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 2: 225-238.
Prasad, M., Varsheny, R. K., Roy, J. K., Balyan, H. S. and Gupta, P. K. 2000. The use of microsatellite for detection DNA polymorphism, genotype identification and genetic divesity in wheat. Theoretical Applied Genetic, 100: 584-592.
Rahimi, A., Zabet, M., Izanloo, A. and Alizadeh, Z. 2015. The study of genetic diversity in different ecotypes of cumin (Cuminum cyminum L.) in Khorasan province (Northern, Razavi and Southern) with protein marker. The new findings Conference on Environment and Ecosystems Agriculture, Tehran University.
Rao, R. V. and Hodgkin, T. 2002. Genetic diversity and conservation and utilization of plantgenetic resources. Plant Cell Tissue and Organ Culture, 68: 1-19.
Rauf, R., Dasilva, J. A. T., Khan, A. A. and Naveed, A. 2010. Consequecea of plant breeding on genetic diversity. International Journal of Plant Breeding, 4: 1-21
Roder, M. S., Plaschke, J., Konig, S. U., Borner, A., Sorrells, M. E., Tanksley, S. D. and Ganal, M. W. 1995. Aabundance, variability and chromosomal location of microsatellites in wheat. Molecular Genetics and Genomics, 246: 327-333.
Roder, M. S., Victor, K., Wendehake, Z. K., Plaschke, J., Tixier, M. H., Leroy, P. and Ganal, M. W. 1998. A Microsatellite map of wheat. Genetics, 149: 2007-2023.
Rohlf, F. J. 2002. Introduction to the exploration of multivariate biological data. Quarterly Review of Biology, 77: 50-51.
Saghai-Maroof, M. A., Blyashev, R. M., Yang, G. P., Zhang, Q. and Allera, R. M. 1994. Extraordinarily polymorphic microsatellite DNA in barely: species diversity, chromosomal location and population dynamics. Proceedings of the National Academy of Sciences. USA, 91: 5466-5470.
Salamati, M. S. and Zeinali, H. 2013. Evaluation of genetic variation in different populations of Cuminum cyminum L. using morphological traits. Iranian Journal of Medicinal and Aromatic Plants, 29(1): 51-62.
Seidler-Łożykowska, K., Kuczyńska, A., Mikołajczyk, K., Nowakowska, J. and Bocianowski, J. 2014. Estimation of genetic distance among genotypes of caraway (Carum carvi L.) using RAPD-PCR. Acta Scientiarum Agronomy, 36(2): 183-188.
Senior, M. L. and Heun, M. 1993. Mapping maize microsatellites and polymerase chain reaction confirmation of the targeted repeats using a CT Primer. Genome, 36: 884-889.
Sepahvand, N. A., Sarhangi, M., Mehrabi, R. and Mostafavi, Kh. 2015. Study on genetic diversity of quinoa morphotypes using microsatellite molecular markers. Modern Genetic, 10(1): 115-122.
Shankaracharya, N. B. and Shankaracharya, M. L. 1988. Research note on the essential oils of Cuminum cyminum L. and Bunium persicum. B. Perfumes and Flavours Association of India Journal, 10(4): 33-35.
Stachel, M., Lelley, T., Grausgruber, H. and Vollman, J. 2000. Application of microsatellites in wheat (Triticum aestivum L.) for studying genetic differentiation caused by selection for adaptation and use. Theoretical and Applied Genetics, 100: 242-248.
Tautz, D. and Renz, M. 1984. Simple sequence are ubiquitous repetitive components of eukaryotic genomes. Nucleic Acids Research, 12: 4127-4137.
Wu, K. S. and Tanksley, S. D. 1993. Abundance, polymorphism and genetic mapping of microsatellites in rice. Molecular Genetics and Genomics, 241: 225-235.
Zargari, A. 1987. Medicinal Plants (Volume 2), Tehran University Press. pp. 761-766.
Volume 8, Issue 2
September 2017
Pages 59-71

Files

Share

How to cite

Statistics