Study of Genetic Differences of Grapevine (Vitis vinifera L.) cv. Bidaneh Sefid Clones Using SSR and AFLP Markers

Document Type : Research Paper

Authors

1 Scientific staff of Agriculture and Natural Resources Research Center of West Azerbaijan, Uromiyeh

2 professor, Department of Horticulture, Tabriz University, Tabriz

Abstract

Clonal selection has become the most important way to improve the quality of grape cultivars. Nowadays, there is a need for reliable and precise methods to characterize of clones for use by nurseries and breeders. To assess the genetic differences among clones of grapevine (Vitis vinifera L.) cv Bidaneh sefid, 10 selected clones from a clonal selection program were analyzed by 23 Simple Sequence Repeats (SSR) and seven Amplified Fragment Length Polymorphism (AFLP) primer combination. No intra-varietal differences could be detected by SSRs among clones whereas eight out of the 499 AFLP fragments generated by the seven primer combinations were polymorphic. The number of markers ranged from 44 (E34-M34) to 97 (E31-M32) with an average of 71.3 fragments per primer combination. Cluster analysis based on AFLP data were separated all the clones of Bidaneh sefid in two groups. The first group includes nine white berry skin clones, without any genetic differences and the second group with only a red berry skin clone. AFLP could only differentiated the red berry clone from other white berry clones.

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