Identification of Microsatellite Markers Linked to Fusarium Head Blight Resistance Genes in Wheat

Document Type : Research Paper

Authors

1 M.Sc. student, Faculty of Agriculture, Bu-Ali Sina University, Hamedan

2 Assosiate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Bu- Ali Sina, Hamedan

3 Assosiated Professor, Agricultural Biotehnology Research Institute of Iran, Karaj

Abstract

In order to identify the molecular markers Linked to Fusarium head blight resistance genes in wheat, F3:4 and F3:5 populations derived from crossing between cv. Falat (susceptible) and cv. Sumai3 (resistant) were evaluated, during two years in farm conditions, under artificial infection. Disease development was measured by area under the disease progress curve (AUDPC). The parental polymorphism was assessed using 170 SSR primer pairs. The 41 polymorphic primer pairs were selected for genotyping of F3:4 individuals. The phenotypic results indicated that, parents and the segregating populations had significant differences for FHB resistance. The best segregation for the resistance was observed in 22 to 26 days after the initial infection. The results of molecular analysis indicated 2 QTLs related to disease resistance, on short arms of 3B and 5B chromosomes, explained 16 % of the phenotypic variation for AUDPC.

Keywords

Volume 3, Issue 1 - Serial Number 1
November 2012
Pages 17-22

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