Document Type : Research Paper
Authors
1
Graduated of Animal Science Department, Tehran University, Karaj, Iran. And Assistant Professor of Animal Science Department, Bu-Ali Sina University, Hamedan, Iran
2
Assistant Professor, Department of Animal Science, Tehran University, Karaj, Iran
3
Professor, Department of Animal Science, Tehran University, Karaj, Iran
4
Associate Professor, Department of Animal Science, Tehran University, Karaj, Iran
5
Professor, Department of Genetics and Biotechnology, Faculty of Agricultural Science, Aarhus University, Tjele, Denmark
Abstract
Pure, sensitive and high quantity and quality extracted DNA is very important in biological research. In this research, the whole blood samples of 600 quails (Quturnix japonica) were used to extract genomic DNA by modified salting out method (with washing powder and without Proteinase K enzyme). Quantity and quality of extracted DNA samples were assayed by Nanodrop, Fluorometer, Bioanalyzer, electrophoresis on gel, digestion with endonuclease enzymes, PCR, barcode and adapter ligation in library making processing and genome sequencing at the end. The averages of extracted DNA per sample and 260/280 OD ratio were 138µg and 1.87 respectively. All of the used DNA samples were successful in amplification, digestion, barcode and adapter ligation and genomic sequencing processes. The results showed that DNA samples were high quantity and quality, sensitive, stable and minimum contamination. In conclusion, DNA extraction by using this method is fast, safe, low cost and simply practical in any lab.
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