Document Type : Research Paper
Authors
1
PhD Student, Department of Agronomy Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
2
Associate Professor, Department of Agronomy Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
3
Associate Professor, Department of Plant Breeding and Biotechnology, Plant Production College, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Abstract
Plant can be produced through the production of a wide range of antimicrobial compounds, including oxidizing enzymes such as Polyphenol Oxidase (PPO) and Peroxidase (POX) protect against phytopatogenic agents. In order to identify the role of these enzymes in maize resistance to fungus F. verticillioides, the experiment was conducted with three corn genotypes with different phenotypes for tolerant to fusarium ear rot consist of C7 (resistant line), B73 (semi resistant line) and MO17 (susceptible line) in three replications. Inoculating corn silk and kernel with Fusarium suspension and they were sampled at 12, 24, 48, 72 and 96 hours after inoculation. Uninfected ears were considered as controls. After evaluating the expression of PPO and POX genes using the Real Time PCR method, it was found that the expression of these two genes increased after maize Fusarium inoculation, but the expression of the gene in the resistant line during the first hours of inoculation was observed higher than the sensitive and semi-resistant lines. In the early hours of inoculation, the resistant line of PPO and POX gene expression in the silk sample was more than the sensitive line, in contrast, the sensitive line of PPO and POX gene expression in the kernel sample was more than the resistant line. However the resistant line showed a higher ability to respond to the disease and immediately after inoculation, the expression of PPO and POX genes was shown. It can be said that probably the importance of PPO and POX genes in the first hours of inoculation that causes the plant's resistance to pathogen entry.
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