Document Type : Research Paper
Authors
1
MSc Graduated, Department of Molecular Plant Breeding, Faculty of Agricultural and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
2
Professor, Department of Molecular Plant Breeding, Faculty of Agricultural and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
3
Associate Professor, Department of Molecular Plant Breeding, Faculty of Agricultural and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
4
Associate Professor, Department of Water Engineering, Faculty of Agricultural and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
5
Associate Professor, Department of Food Science and Technology, Faculty of Agricultural and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
Abstract
Water deficit is one of the most important abiotic stresses constraining crop production in rapeseed. Understanding the mechanisms of adaptation to this stress is essential for the development and production of drought tolerant cultivars. In this sense, this research study aims to investigate the importance of genetic diversity in identifying cultivars with a high degree of drought tolerance through assessing effectiveness of Inter Simple Sequence Repeats (ISSR) markers on 14 cultivars of rapeseed in a factorial design based on a randomized complete block design with three replications. Morphological and physiological characteristics were studied after the early stages of culture. In order to evaluate the genetic diversity among cultivars, 18 different ISSR markers were used. A total of 106 clear and scalable loci were amplified, of which 60 bands (56.6%) were polymorphic. Polymorphism information content (PIC) belonged to marker number 9 with the amount of 0.365 (85.7%). Gene variation ranged from 0.081 to 0.365. The rapeseed cultivars were divided into 3 groups by cluster analysis (UPGMA method). The analysis of molecular variance showed that 70% of the total variation was observed within populations and 30% of this variation occurred among populations. Regarding the content of seed oil in the control group, Adriana had the highest amount of oil with 36.47%, whereas Karaj 2 had the lowest amount with 27.28, Cooper had the highest decrease in oil content percentage under stress conditions.
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