Agricultural Biotechnology

Agricultural Biotechnology

The Effect of Glyphosate on Transgenic Potato Plant Harboring aroA Gene and Induction of Systemic Acquired Resistance Towards Two Bacterial Pathogens

Document Type : Research Paper

Authors
Hossein Pasalari
Chamran Hemmati
Assistant Professor, Department of Agriculture , Plant Protection Research Group, Minab Higher Education Complex, Hormozgan University, Bandar Abbas, Iran
10.22084/ab.2021.20915.1426
Abstract
Our objective was to investigate the effect of glyphosate in induction of resistance to two plant bacterial pathogens. To do so, glyphosate at an optimal concentration of 1.8 mg / l was used on the transgenic potato, Odyssay cultivar, to induce resistance to two strains of pathogenic bacteria (21A of Pectobacterium atrosepticum and ENA49 of Dickeya dadantii). Transgenic potato leaves infected with potato pathogenic bacteria, and then treated with glyphosate showed a high level of expression of pathogenesis-related genes (PR-2, PR-3, PR-5), especially PR-2 and defense response genes (HSR-203j, HIN1), especially HSR-203j. The expression of PR-2 gene in leaves infected with these two bacteria were 1.5 and 2.9 times for PR-3 gene, 1.7 and 1.7 times for PR-5 gene to 1.3 and 1.5 times and expression of HSR-203J gene to 2.5 and 2.4 times and for HIN1 gene to 1.7 and 1.7 times, with Dickeya dadantii and Pectobacterium atrosepticum infection, respectively. However; the plants infected with bacteria and non-treated by glyphosate did not significantly change the expression of these genes. The results showed that the treatment of plants by glyphosate may not only eliminate weeds of farmland but can also induces a systemic acquired resistance to pathogenic bacteria by expressing of PR proteins and defense response genes.
Keywords
Transgenic potato
Bacterial pathogens
Glyphosate
Potato pathogenesis related and defense response Genes
Systemic acquired resistance
Subjects
Agricultural Biotechnology
Volume 11, Issue 2
March 2021
Pages 51-56