Document Type : Research Paper
Authors
1
PhD Student, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran
2
Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran
3
Assistant Professor, Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqhiyatallah University of Medical Sciences, Tehran, Iran
4
Assistant Professor, Dryland Agricultural Research Institute (DARI), Agriculture Research, Education and Extension Organization (AREEO), Maragheh, Iran
5
Dryland Agricultural Research Institute (DARI), Agriculture Research, Education and Extension Organization (AREEO), Maragheh, Iran
Abstract
Abiotic stresses, such as drought, affect the growth and development of plants like common bean (Phaseolus vulgaris L.) and plant responses to them are associated with large variations in complex gene networks. In the present study, changes in gene expression pattern under drought and normal conditions of common bean were studied through bioinformatics analysis of ESTs (expressed sequence tags) data from the NCBI database. After initial splicing, EST sequences were clustering and assembling, resulting in 8983 unigene under normal and 6665 unigene of drought stress conditions. Grouping and genetic enrichment analysis the common bean library EST sequences under drought stress based on biological processes, molecular function and cellular component at 23, 16 and 20 different functional groups, respectively. Protein-protein interaction network showed that genes encoding antioxidant enzymes (CAT1, APX1, APX3, APX1B, GSTU25), Proline-rich proteins (P5CS1, P5CS2), heat shock proteins (ATHSP70, HSP70b, ATHSP90.1, ATHSP90.5) and ubiquitin family proteins (UBQ3, UBQ11) account for a large share of the regulatory network in starvation water. Additionally, we found that the expression of photosynthetic genes decreased. In this study, provides a list of transcription factors (including: RAP2.10, DEAR2, NF-YC9, WRKY40, ARF1, CDF2, ATCTH, ERF-1) as one of the excellent point that have not been reported with so many detail in the common bean so far and it can be very important and applicable. The genes identified in this study could be suitable candidates for drought resistance and marker-assisted selection in common bean.
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