Document Type : Research Paper
Authors
1
MSc Graduated, Department of Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamadan, Iran
2
Associate Professor, Department of Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamadan, Iran
3
Professor, Department of Plant Protection , Faculty of Agriculture, Bu- Ali Sina University, Hamadan, Iran
Abstract
Different genera of fungi coexist in Elaeagnus angustifolia plant. These fungi are able to produce some valuable extracellular enzymes, from a research and economic perspective. In this research, the presence of endophytic fungi and some of their enzymatic properties were investigated in Elaeagnus angustifolia tree. Sampling was performed from healthy tissues (leaf, fruit, Branch and bark) of Elaeagnus angustifolia trees of Hamedan city Initial identification of fungi was conducted using fungal identification keys. After isolation and purification, endophytic fungi were examined for the presence of some extracellular enzymes. Forty seven fungal isolates of different genera, such as Aspergillus, Penicillium, Fusarium, and Alternaria were isolated. The results showed that the activity of proteases, cellulase, pectinase and catalase enzymes of different fungi were different. No lipase activity was proven in these fungi. Amylase activity in these fungi showed that the enzyme activity varied depending on the type of consumed substrate. This is the first report of isolation of fungal endophytes of Elaeagnus angustifolia tree.
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