Document Type : Research Paper
Authors
1
MSc Student, Department of Plant Production and Genetics, Faculty of Science and Agricultural Engineering, Razi University, Kermanshah, Iran
2
Professor, Department of Plant Production and Genetics, Faculty of Science and Agricultural Engineering, Razi University, Kermanshah, Iran
Abstract
Black cumin is belong to Ranunculaceae family that it has important secondary metabolites especially thymoquinone that used in the pharmaceutical industry. The purpose of this study was optimization of conditions for callus induction and cell suspension culture with use salicylic acid and methyl jasmonate elicitors to enhance some important secondary metabolites, especially thymoquinone. Callus induction experiment was carried out in factorial arrangement within CRD template with three replications. Three factors included explants, BAP and IAA. Cell suspension experiments were carried out in completely randomized design (CRD) with two replications with used of stem callus of the best plant growth regulator. In the first experiment of cell suspension culture, salicylic acid in four concentrations (0, 125, 250 and 500μM) and in the second experiment, methyl jasmonate in five concentrations (0, 50, 100, 200 and 300μM) were used and the results of the cell suspension for secondary metabolites were analyzed by GC-MASS. The results of callus induction experiments showed that the best explants for callus induction (100%), was stem in combination with 1/5 mg/l BAP and 2mg/l IAA. The results of cell suspension experiments showed that in both of the cell suspension experiments, different concentrations of elicitors have different effects on the increasing or decreasing in some secondary metabolites. The results indicated that thymoquinone content was maximally increased by 33% times, in comparison to control, when salicylic acid (500μM) were added and maximally increased by 43% times, in comparison to control, when methyl jasmonate (300μM) were added as abiotic elicitors.
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