Document Type : Research Paper
Authors
1
Former Student, Department of Horticultural Sciences , Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
2
Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran
3
Assistant Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran
Abstract
In this study the the genetic diversity and population structure of six population of hyssop was evaluated using ISSR markers. The evaluated populations included 5 native populations from Iran (Arak, Shiraz, Mashhad, Isfahan1, Isfahan2) and one population from Sweden. Eighteen primers were analyzed resulting in 126 high-resolution bands, among which 119 bands were polymorphic. Cluster analysis on the basis of Jaccard genetic similarity matrix and UPGMA method divided hyssop samples into 6 groups. The method of Principal coordinate analysis (PCOA), confirmed the results of cluster analysis to a great extent. The highest genetic similarity was observed between Isfahan 1 and Mashhad populations and the lowest between Arak and Sweden populations. Diversity within populations was analyzed using the mean of Nei’s gene diversity Index (h) and Shannon Information Index (I). The highest and lowest genetic diversity within the population were observed in Isfahan 1 (h = 0.24 and I = 0.36) and Mashhad (h = 0.14 and I = 0.21), respectively. The mean of effective alleles (Ne) to observed alleles (Na) was 0.85. Based on the results of molecular analysis of variance (AMOVA), the mean indices of Gst (gene diversity) and Dst (genetic diversity between populations) were 0.38 and 0.12, respectively, indicating high genetic differentiation between populations. Molecular analysis of variance between populations also showed that 61% of the total genetic diversity in the samples was related to diversity within populations and 39% among populations. In the study of the genetic structure of the populations using STRUCTURE software, the populations were divided into four groups which Swedish population was the purest one. In this study, the employed ISSR markers showed high percentage of polymorphisms and were able to differentiate populations well. So that, this method is suggested to describe genetic differences within and among hyssop populations.
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