Document Type : Research Paper
Authors
1
PhD Student, Department of Plant Protection, Faculty of Agriculture, Razi University, Kermanshah, Iran
2
Professor, Department of Plant Protection, Faculty of Agriculture, Razi University, Kermanshah, Iran
3
Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Razi University, Kermanshah, Iran
4
Assistant Professor, Department of Agronomy, Faculty of Agriculture, Razi University, Kermanshah, Iran
Abstract
Green Lacewing as one of the most important groups of insects with characteristics such as the possibility of mass production, geographical distribution and host range have been considered in biological control. The study about genetic diversity is one of the effective tools in genetic conservation of important species. Today, the production of barcode sequences for species identification is expanding. Mitochondrial genes are important tools for various studies in the fields of population genetics, evolution and phylogenetics. This study investigated the genetic diversity of populations of this species from seven provinces of Iran, using part of the mitochondrial DNA gene, cytochrome oxidase COI. Gene sequences of the samples were compared using MEGA5 and Bioedit7 softwares. The obtained sequences were analyzed with 517 bp for mitochondrial cytochrome oxidase I (COI) gene. Genetic parameters related to 9 haplotypes in these populations were examined. Haplotypic diversity, number of nucleotide differences and nucleotide diversity in all populations were calculated as 0.91429, 1.93333, 0.00374, respectively. The highest nucleotide diversity was observed for Gilan and Kermanshah provinces, the highest number of nucleotide differences was observed in the population of East Azarbaijan province and the highest haplotypic diversity was observed in the population of Kermanshah, Gilan and East Azarbaijan provinces. The results of geographical grouping showed that the southern regions of the country have less haplotypic diversity than other regions.
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