Document Type : Research Paper
Authors
1
MSc Student, Department of Plant Production and Genetics, Faculty of Science and Agricultural Engineering, Razi University, Kermanshah, Iran
2
Professor, Department of Plant Production and Genetics, Faculty of Science and Agricultural Engineering, Razi University, Kermanshah, Iran
3
Assistant Professor, Department of Plant Production and Genetics, Faculty of Science and Agricultural Engineering, Razi University, Kermanshah, Iran
Abstract
Lallemantia iberica belongs to the family of Lamiaceae, a year-old herb that has medicinal and oil properties. For optimization of tissue culture of this plant, two experiment: callus induction and regeneration were done. In order to investigation of callus formation, a factorial experiment was conducted in a completely randomized design with three factors: explant types (hypocotyl and cotyledon), BAP growth regulator (0, 0.5, 1, 1.5 mg/l) and NAA growth regulator (0, 1, 2, 4 mg/l) with three replications. Results of analysis of variance (ANOVA) for callus formation traits showed significant differences between some of the studied factors. The results of mean comparison of interaction effects between the studied factors showed that the highest rate of callus formation (100%) in the hypocotyl explant in NAA (4 mg/l) and BAP (0 mg/l) treatment and for cotyledon explant (100%) with the combination of BAP (1.5 mg/l) and NAA (0 mg/l) were obtained. In regeneration experiment four levels of BAP (1, 2, 3, 4 mg/l) was used. The highest percentage of regeneration was obtained for hypocotyl explant (70%) in 4 mg/l BAP and for cotyledon explant the highest percentage of regeneration (26.66%) was obtained in 3 mg/l BAP treatment. Due to the importance of this plant results of tissue culture optimization, could be use for increasing secondary metabolites and genetic engineering researches.
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