Comparative Analysis of Four DNA Extraction Protocols for Fresh and Dried Specimens of Five Medicinal Lamiaceae Plants

Document Type : Research Paper

Authors

1 PhD Student, Department of Horticultural Sciences. Faculty of Agriculture,, University of Bu-Ali Sina. Hamedan, Iran

2 Assistant Professor, Faculty of Medicinal Plants, Amol University of Special Modern Technologies, Amol, Iran

3 Assistant Professor, Department of Horticultural Sciences. Faculty of Agriculture,, University of Bu-Ali Sina. Hamedan, Iran

Abstract

Extraction of genomic DNA of high quality and quantity from some medicinal plants is difficult. It can be attributed to the presence of high amounts of specialized metabolites biosynthesized into their cells. On the other hand, molecular biological studies require successful extraction of high-quality DNA. Hence, the present study aimed to compare four modified procedures of DNA extraction including Murry and Thompson (1980), Dellaporta et al. (1983), Doyle and Doyle (1990) and Ziegenhagen et al. (1993) from five genera of Lamiaceae. Fresh and dried leaves of Rosmarinus officinalis, Lavandula officinalis, Salvia hydrangea, Dracocephalum kotschyi and Ziziphora tenuior were used for the procedures. Following measurement of the extracted DNA quantity and quality, the DNA preparations were tested by PCR amplification using two inter-simple sequence repeats (ISSR) primers. All of the procedures were capable of extracting DNA from five genera. The modified Murray and Thompson method was found to be the most efficient DNA extraction method, indicating high DNA concentration (570.57ng/µl), good-quality DNA (1.80), affordable cost and less time. The results also showed that the quality and yield of extracted DNA from fresh leaves were relatively higher than those of dried leaves. In addition, banding pattern obtained using 1% agarose gel and PCR showed the same results. According to the data, Murray and Thompson method is recommended for DNA extraction from fresh and dried leaves of five studied medicinal plants.

Keywords

Volume 11, Issue 1
April 2020
Pages 1-10

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