Evaluation of Proteins Expression Differences of Royan Axes in Two Cultivar of Rice with Two-dimensional Electrophoresis

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Agriculture (Plant Breeding and Genetics), Payame Noor University, Tehran, Iran

2 MSc, Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Abstract

The aim of this study is to evaluate and comprehensively quantify the molecular changes created in the expression of rice embryo axes proteins by two-dimensional electrophoresis method. The variability in the protein pattern of the embryonic axes was evaluated in two rice tolerant and salinity-sensitive cultivars (Hassani and Sangjoo, respectively) during three replications. As result of the analysis of two-dimensional electrophoresis gels in the rice embryo axes, 328 repeatable spots were observed, which 63 spots showed a significant difference at the level of 1%. Multivariate statistical methods, such as cluster analysis and diagnostic function analysis, were used to group the stains expressed in Sangjoo and Hassani cultivars. The significant difference between the two cultivar showed that the tolerable cultivar they had the characteristic of stress tolerance. Cluster analysis placed proteins -in terms of expression- in three main clusters, indicating the presence of proteins with similar expression in each cluster. This indicates the similar performance of the proteins in each cluster and the presence of all of them in a common biological pathway. Discriminant analysis confirmed the results of clustering analysis 100% approved and indicating that the protein data are consistent with the experimental conditions. Due to the higher number of expression stains in Hassani's tolerance to salinity stress, and the decrease in stain expression in sensitive Sangjoo cultivar, it is possible to suggest the identification of genes related to these proteins. Overall, the results showed that using statistical software and analysis, it is easy to evaluate significant expression changes due to differences in the cultivar fetal proteome levels and can to determine the index of changes.

Keywords

Volume 10, Issue 1
September 2019
Pages 43-53

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