Document Type : Research Paper
Authors
1
Assistant Professor, Department of Agronomy and Plant Breeding, Young Researchers and Elite Club, Kermanshah Branch, Islsmic Azad University, Kermanshah, Iran
2
MSc Student, Department of Agronomy and Plant Breeding, Young Researchers and Elite Club, Kermanshah Branch, Islsmic Azad University, Kermanshah, Iran
3
Professor, Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract
Because of to the importance of wheat, it seems necessary to identify the proteins involved in different stages of its growth. This is for breeding more capable plants, which have more production and are resistant to adverse environmental conditions. In this study, proteomics was used to identify and compare the protein pattern of bread wheat in developmental stages of leaves and tillering stages. For this purpose, Bread wheat cultivar Azar2 was cultivated in greenhouse conditions and samples were taken at different stages of leaf development (single-leaf, two-leaf and three-leaf) and three stages during the tillering period. Protein extraction was performed by TCA-acetone method and proteins were separated by 2D electrophoresis. By analyzing the resulting gels by Image master 6.0 software, 360 reproducible protein spots were identified in the leaf development stages and 400 reproducible protein spots in the tillering stages. Of these, 31 protein spots in different stages of leaf development and 31 protein spots in different stages of tillering showed significant changes. Finally, protein spots were identified based on their molecular weight, isoelectric point, spot shape, and by searching related articles and the Uniprot site. The results showed that in these stages, a wide range of metabolic activities including photosynthesis, respiration, protein biosynthesis, protein transport, protein assembly, and packaging, carbohydrate biosynthesis, message transmission system, toxin degeneration and basic genetic processes (replication, transcription and translation) were changed during different stages of wheat plant growth.
Keywords
)