Document Type : Research Paper
Authors
1
MSc Student, Department of Agrotechnology, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
2
Associate Professor, Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
3
Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
4
Assistant Professor, Department of Production Engineering and Plant Genetic, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract
Bentazone is a postemergence herbicide used to control weeds in bean fields. This study aimed to isolate and identify bentazone degrading bacteria in some areas of Selseleh city. According to the results of phenotypic and biochemical tests, 30 isolates of bentazone herbicide-degrading bacteria were divided into four phenotypic groups, including Bacillus, Pseudomonas, Acinetobacter, and Stenotrophomonas. Highly degrading isolates including BaBLU3 in the Bacillus group, PsBLU2 in the Pseudomonas group, StBLU5 in the Stenotrophomonas group, and AcBLU2 in the Acinetobacter group were selected, according to the results of the minimum inhibitory concentration and the minimum bactericidal concentration. The sequencing results of housekeeping gene 16S rRNA, revealed the BaBLU3, PsBLU2, StBLU5, and AcBLU2 isolates belonged to the B. altitudinis, Pseudomonas sp., Stenotrophomonas sp. and A. calcoaceticus, respectively. This study is the first report of in vitro bioassay of the Bentazone herbicide biodegradation by using the isolates of B. altitudinis, Pseudomonas sp., Sthenotrophomonas sp. and A. calcoaceticus. Due to the ability of these bacterial isolates in degrading Bentazone herbicide, their use in bioremediation research to remove the remains of this herbicide in bean fields is recommended.
Keywords
Main Subjects
)