Document Type : Research Paper
Authors
1
PhD Student, Department of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2
Associate Professor, Department of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3
Associate Professor, Department of Vegetable and Irrigated Pulse Crops Research, Seed and Plant Improvement Institute, Karaj, Iran
4
Associate Professor, Department of Plant Protection, Faculty of Agriculture, Zanjan University, Zanjan, Iran
Abstract
Tomato spotted wilt virus disease is one of the most important tomato diseases that causes damage in the country. To identify inbred resistant lines against Tomato spotted wilt virus (TSWV) in the present study, 64 tomato genotypes including 28 tomato hybrid (F1) cultivars were initially evaluated for the presence of Sw-5b conferring resistance using a codominant SCAR (Sequence characterized amplified region) molecular marker, NCSw003. Based on the results obtained from this stage of the study, only four tomato hybrid cultivars namely Sereen, Brivio, Jam and Xaman, carried the resistance allele. Based on the results of the second stage and 240 selected F6 inbred lines with appropriate agronomical traits those were generated from four the above-mentioned heterozygous cultivars, The inbred lines generated from Sereen, Jam, Xaman and Brivio carried out 35.6, 35.1, 25.7 and 22.2 percent homozygous resistant allele specific, respectively. Furthermore, single nucleotide polymorphism in Sw-5b gene allele was investigated for F6 selected progenies of three heterozygous cultivars, Sereen, Xaman, and Brivio using high resolution melting (HRM) technique. The comparison between the results of screening by HRM and NCSw-003 codominant SCAR marker showed 86, 98.6 and 91 consistencies, respectively. Considering identical results of both markers used for each of the above cultivars, 21, 18 and 12 pure homozygous resistant lines were identified, respectively. Also, the results of the study using NCSw-003 marker alone showed that 20 pure lines obtained from Jam cultivar have dominant homozygous resistance. The identified resistance lines with homozygous resistance can be used after biological evaluation as a resistant parent in tomato breeding programs and introduction of hybrid cultivar.
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