Investigation of Genetic Diversity of Pyrus Cultivars Using Microsatellite Molecular Marker (SSR)

Document Type : Research Paper

Authors

1 MSc Graudate, Department, of Horticultural Sciences, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

2 Assistant Professor, Department of Biology, Faculty of Basic Sciences, Lorestan University, Khorramabad, Iran

3 Associate Professor of Department of Horticulture, Seed and Plant Improvement institute, Karaj, Iran

Abstract

In order to study the genetic diversity and grouping among 20 cultivars (from two species) of Pyrus from eastern and western species in the Research Institute of Breeding and Preparation of Seedlings and Seeds of Karaj based on 12 pairs of primers were evaluated with molecular marker (SSR). The markers used produced a total of 273 bands with a size of 100 to 150bp and, on average, each primer combination produced 6.46 polymorphic bands. The average of 90.33% for polymorphism, 0.654 polymorphic information content (PIC), 0.962 Shannon information index and 2.69 marker index indicated the existence of high genetic diversity of cultivars. Cluster analysis of molecular traits based on Dice similarity coefficient divided the cultivars into four groups. Changes in Dice similarity coefficient between 0.58 and 0.95 indicated the existence of high genetic diversity among the studied cultivars. Principal component analysis showed that the first three components together accounted for 54.50% of the total genetic diversity. Based on the results of the present study, four primers BGT23b, NH030a, NB103a and NH036b can be introduced as useful and desirable primer compounds for cultivar differentiation, so that it can be concluded that this group of primers can be used in screening the population of pear cultivars.

Keywords

Volume 12, Issue 2
March 2022
Pages 1-9

Files

Share

How to cite

Statistics