Micropropagation of Wild Pear (Pyrus syriaca)

Document Type : Research Paper

Authors

1 Research Assistant Professor, Research Center for Agriculture and Natural Resources of Fars Province, Shiraz

2 Master of Agricultural Biotechnology, Faculty of Agriculture, Zabol University, Zabol

3 Master of Agricultural Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamedan

Abstract

Fars province is one of the natural habitats of Pyrus syriaca in Iran. Harvesting of fruits, deforestation, expansion of agriculture and overgrazing in recent years imposed detrimental effects on natural regeneration of this species.This research was carried out to develop tissue culture techniques for easy, rapid and mass propagation of P. syriaca. Shoot tips and nodal segments of current season growth shoots of selected trees in Deh-Kohne open forest in Sepidan County, Fars province, Iran were used as explants. Cultures free of contamination were established on MS medium containing 0.4 mgl-1 BA, 0.01 mgl-1 IBA. Concentration of 0.8 or 1.0 mgl-1 BA was optimum for shoot multiplication. The best procedure for rooting of in vitro produced shoots consisted of two phases: in vitro root induction and ex vitro root development. For root induction, P. syriaca shoots were cultured on DKW medium (half strength macronutrients) containing 75 mgl-1 IBA and incubated in the darkness for 24 hours. For root development, shoots were transferred from root induction medium to moistened Jiffy-7 pots. Using this method 100 percentage of shoots rooted. Inclusion of 0.1 mgl-1 BA in nutrient media had negative effects on rooting of P. syriaca shoots and significantly decreased rooting percentage.

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