Expression of Some Defensive Genes Responsible for Direct and Indirect Defense Against Spider Mite, Tetranychus urticae Koch (Acari: Tetranychidae), in Some Common Bean Cultivars

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam

2 Associate Professor, Department of Agronomy and Plant Breeding, University of Agriculture and Natural Resources, University of Tehran, Karaj

3 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Gorgan University of Agriculture and Natural Resources, Gorgan

Abstract

Herbivore feeding induces two main defensive systems in plant, direct and indirect defenses. To know role of some genes which involve in direct and/or indirect defenses their expression pattern were compared in clean and mite infested leaves of some common bean susceptible (Akhtar) and resistant (Naz, Ks41128) genotypes with QRT-PCR. Then to proof the QRT PCR results, preference of predatory mite, Phytoseiulus persimilis، were compared in all cultivars with olfactometer. The QRT-PCR results indicated that among genes only expression of LOX gene that is important in both direct and indirect defenses was significantly difference among genotypes. After spider mite infestation expression of LOX gene was significantly up-regulated only in the resistant genotype (Naz) and in the susceptible genotype (Akhtar) expression of LOX was even significantly down-regulated. The result of olfactory tests showed that only Naz genotype could significantly attract more predatory mites after the spider mite infestation. Therefore significantly more attraction of predatory mite to resistance genotype (Naz) than other genotypes and up regulation of LOX gene in this genotype upon mite infestation can demonstrate role of this gene in direct and indirect defense of common bean against spider mite.

Keywords

Volume 3, Issue 2 - Serial Number 2
December 2013
Pages 9-16

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