Effects of Plant-Derived Smoke and Gibberellic Acid on Seed Dormancy Breaking, Seed Germination Traits and Seedling Growth of Rapeseed (Brassica napus L.) Under in Vitro Condition

Document Type : Research Paper

Authors

1 M.Sc student, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan

2 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan

3 Associate Professor, Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modares University, Tehran

Abstract

The effect of aqueous smoke extract derived from burning of Tanacetum parthenium leaves and GA3 on secondary dormancy breaking, germination and seedling growth of rapeseed seeds (Topaz and PF704 cultivars)was investigated in this study. In the first experiment the rapeseed seeds of two cultivars imbibed with different concentrations of aqueous smoke extract (0.0005, 0.001, 0.002, 0.004, 0.01, and 0.1 (v/v)) and distilled water (control) were investigated as a completely randomized factorial 2×7 with 3 replications. In second experiment difference concentrations of aqueous smoke extract (0.001, 0.002, 0.004, 0.01) and control (with out smoke water) combined with difference concentrations of GA3 (0, 0.1, 0.15, 0.20 mgl-1) were used in B5 regeneration medium. The experiment was arranged in a completely randomized design, factorial 5×4 with 3 replications. In the first experiment, use of aqueous smoke extract (concentration of 0.1) showed the highest germination percentage and shoot length in dormant seeds of both cultivars, while this concentration showed the highest root length in Topas cultivar. Use of GA3 (concentration of 0.1 and 0.20 mgl-1) in combination with aqueous smoke extract (0.1) showed the highest germination percentage in Topas, while use of GA3 (concentration of 0.1 and 0.15 mgl-1) combined with all aqueous smoke extract concentrations indicated the highest germination percentage in PF704 cultivar. In both cultivars, two concentrations of aqueous smoke extract (0.004 and 0.01) combined with GA3 (concentration of 0.1) in culture medium showed the highest shoot length and root length.

Keywords

Volume 3, Issue 2 - Serial Number 2
December 2013
Pages 17-26

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