Biological Control of Strawberry Root Rot Using some Isolates of Trichoderma harzianum

Document Type : Research Paper

Authors

1 PhD Student, Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

2 Associate Professor, Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

3 Professor, Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

4 Professor, Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

Abstract

Black root rot disease occurs as a limiting factor of strawberry production worldwide, which is primarily caused by the Rhizoctonia spp. and several other pathogens. One of the ways to management this disease is biological control using Trichoderma species. Aim of this study was to assess Trichoderma harzianum isolates from Khorramabad County, for their ability to control of strawberry root rot disease caused by Rhizoctonia solani in vitro and in vivo and their ability to promoting growth of strawberry plants. Trichoderma fungal isolates were isolated from the rhizospheric of forest areas of Khorramabad County and were identified based on morphological and phylogenetic analysis of sequence tef1 gene. The results showed that mycelial growth inhibition of R solani by Trichoderma isolates ranged from 41.4% -60.8% in dual culture, LT36 isolate showed the highest impact on mycelial growth inhibition in dual culture. All selected isolates produced volatile metabolites which prevented radial growth of pathogen from 37.9% to 62.3%. The results of greenhouse experiments showed that LT36 isolate increased the dry weight shoot by 192.2%. While LT196 isolate increased 155% and 55.5% dry weight and root length respectively. The lowest disease severity index with 30% was related to LT36 isolate. These treatments significantly increase growth parameters compared with the check treatment. Which proved the ability of Trichoderma as plant growth promoter.

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