Document Type : Research Paper
Authors
1
PhD student, Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Karaj
2
Professor, Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Karaj
3
Associate Professor, Agricultural Biotechnology Research Institute of Iran, Karaj
4
Associate Professor, Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Karaj
5
Laboratory Technician, Agricultural Biotechnology Research Institute of Iran, Karaj
6
Assistant Professor, Azad University, Arak Branch, Arak
Abstract
Septoria tritici blotch (STB), caused by Mycosphaerella graminicola (anamorph Septoria tritici), is one of the most important diseases of wheat (Triticum aestivum) world wide. planting resistant cultivars is the best method to manage STB environmentally. Many plant genes are induced or repressed and many others are up regulated or down-regulated to response a pathogen. In this study, Real time PCR were used to analysis of expression patterns of five genes involved in septoria leaf blotch resistance in wheat. After inoculation of resistant (Wang shui bai) and susceptible (Falat) cultivars with M. graminicola fungus, expression of these genes were investigated at 11 time points in 3 replications and the expression patterns of them were determined as relative to 18s reference gene. Comparision of these sequences using NCBI data base showed that these sequences had homology to neomenthol dehydrogenase, Lucine rich repeat protein kinase, natural resistance-associated macrophage protein, alpha 1,4-glucan phosphorylase and Tubby-like F-box protein. The peak expression for 1,4-glucan phosphorylase and Lucine rich repeat protein kinase were at 3 hours after inoculation and for neomenthol dehydrogenase the peak is at 12h. For natural resistance-associated macrophage protein the peak was at 3h but it was decreased and then increased again at second day. Tubby-like F-box protein was induced at 3h and had significant expression in this time but the peak expression for this gene was at 3 day after inoculation and we can call this pattern, a bimodal one. Some of these genes were induced early after inoculation and their expression increased significantly, but some others showed an induced expression several days after inoculation.
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