Document Type : Research Paper
Authors
1
Former MSc Student, Department of Biology, Faculty of Sciences, University of Zabol , Zabol, Iran
2
Assistant Professor, Department of Biology, Faculty of Sciences, University of Zabol , Zabol, Iran
3
Associate Professor, Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Zabol , Zabol, Iran
4
Associate Professor, Department of Biology, Faculty of Sciences, University of Zabol , Zabol, Iran
10.22084/ab.2024.27377.1485
Abstract
In this study, the anti-diabetic, anti-cancer and antioxidant properties of the hydroalcoholic extract of Ferula gummosa were investigated. Cytotoxicity assay was performed in vitro using the MTT assay on two colon cancer and normal cell lines (HT-29 and HEK293). In the anti-diabetic studies, a murine model of diabetes was utilized in which male rats were treated with streptozocin and in the antioxidant tests, the rat model induced with D-galactose was used. The animals were divided into three groups: healthy, diabetic, and diabetic rats treated with 100 mg/kg/BW of the plant extract of Ferula gummosa. The results showed that the hydroalcoholic extract of Ferula gummosa, in a concentration-dependent manner, significantly decreased the viability of HT-29 cancer cells compared to untreated cells, while the extract had no significant toxicity on normal HEK293 cells (p<0.05). Also, the results of antioxidant tests showed that the activity of catalase and superoxide dismutase enzymes in the liver of rats treated with D-galactose and plant extract increased compared to rats treated with D-galactose, while the levels of malondialdehyde was reduced.The results of in vivo analyses demonstrated a significant decrease in the fasting blood glucose level of diabetic rats treated with the medicinal extract compared to untreated diabetics (p<0.05). Also, histopathological evaluations showed recovery after streptozocin-induced injury and reduced liver cell necrosis in extract-treated diabetic rats compared to untreated diabetic patients. According to the findings of this study, Ferula gummosa medicinal plant extract, which contains antioxidant compounds such as phenols and flavonoids, was able to eliminate colon cancer cells and recover injured liver cells. As a result, in clinical research, the chemicals in Ferula gummosa are presented as potential pharmaceutical candidates with fewer adverse effects. At the same time, more histopathological, biochemical, and molecular research seems to be required.
Keywords
Main Subjects
)