Document Type : Research Paper
Authors
1
PhD. Student, Department of Biology, Kerman Branch, Islamic Azad University, Kerman, Iran
2
Assistant Professor, Department of Biology, Kerman Branch, Islamic Azad University, Kerman, Iran
3
Assistant Professor, Horticulture Crops Research Department, Kerman Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran
4
Instructor, Horticulture Crops Research Department, Kerman Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran
10.22084/ab.2024.28515.1489
Abstract
The date palm is one of the most important trees in dry and tropical regions as a good food source and plays an important role in the country's economy. Therefore, this study aims to investigate the population structure, genetic diversity and protection of the genetic reserves of important date genotypes using the ISSR molecular marker for use in genomic studies. The present research was conducted to evaluate the genetic diversity of 70 Iranian date genotypes belonging to 13 different populations, using 10 ISSR markers. The number of alleles in each gene location was between 5 and 10, and 65 alleles were observed. The highest content of polymorphic information was 30, and the lowest was 6%, respectively, for primers C877 and C842, and the content of polymorphic information was 10 markers with an average of 19.4%. Principal component analysis (PCoA) showed that the two main components included 80.43% of the total changes. The highest genetic similarity based on the Jaccard coefficient was observed between the G12 (Diri) and Deglet-Noor (G13) genotypes, and the lowest value between the male base genotypes (G19) and Amehshanbe (G36), due to genetic diversity in local populations, which can be taken into account in the race. The dendrogram of the cluster analysis based on the UPGMA method showed six main clusters. In this study, some genotypes were not grouped based on geographical distribution. Date male genotypes differed from the other genotypes in both UPGMA and PCoA clustering cases. As a result, suitable approaches of ISSR markers have created distinctions among the 70 date palm genotypes studied, which will provide valuable information for the future improvement of this important product.
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