Study of Allelic Variation of GLU-B1 Locus in some Iranian Durum Wheat Using STS Marker

Document Type : research

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Abstract

Genetic diversity assessment is a feasible approach and useful for germplasm. In this study, 30 genotypes of durum wheat were evaluated for GLU-B1 loci with three specific primer pairs. Nine alleles were present at the GLU-B1 locus. The b (BY8) allele was observed more frequently at the GLU-B1 locus. The amount of genetic variation for each primer that initiates B1.2 (0.85) primer were able to identify polymorphisms more than other primers. Cluster analysis was performed using UPGMA method and the similarity coefficient of 0.62 based on jaccard subdivided the genotypes into eight subgroups. The results revealed that the HMW subunits in durum wheat genotypes are valuable sources and they can be used in breeding program to improve the quality of wheat products.

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