Identification and Study of Genetic Diversity of Botrytis cinerea Isolates Caused Strawberry Grey Mold in Kurdistan Province

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Abstract

Total of nineteen isolates of Botrytis sp. were collected from infected strawberry plants of hydroponic cultivated greenhouse and fields surround kurdistan province. According to the morphological characteristics, isolates were identified as Botryits cinerea species. For confirming the species identification by molecular method specific primers C729 were used and PCR test repeated twice. The results showed that these isolates amplified the 700 bp band which is specific for this species. Genetic diversity of existing isolates was investigated by RAPD analysis. According to the results obtained, isolates were clustered into four groups. Considering this clustering mode, in first glance, most of the greenhouse isolates were grouped together and separated from the group of field isolates, but some of them didn’t follow the pattern and were divided differently. Likewise, study among field isolates collected surround the province and their relationships, showed that there is no correlation between molecular clustering and geographical origin of these isolatesو but also it was clear that some of the geographically further isolates were more similar to each other rather than isolates from closer geographical regions.
 

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References

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