Evaluation of Genetic Diversity in Rapeseed Genotypes Using ISSR Markers

Document Type : research

Authors

1 Graduated M.Sc., Depertment of Agronomy and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam

2 Associate Professor, Depertment of Agronomy and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam

Abstract

Rapeseed (Brassica napus) with more than 40 percent of oil and more of 40 percent of cake protein and just 5 percent of saturated fatty acids in oil is accounted as an important oil grains. Evaluation of genetic diversity is the main and basic principles of each breeding program. In this study genetic diversity among 45 various genotypes investigated by 15 ISSR markers. For analysis of obtained data, amplified bands scored as zero (absent of band) and one (present of band) data with Darwin5.0, MEGA3.1 and Xlstat softwares. Cluster analysis of data obtained by use of Dice dissimilation coefficient and neighbor joining (NJ). Obtain dendrogram divided genotypes into five clusters. Number of all amplified alleles was 110 and equal to average of 7.33 alleles for each marker.Total distance among genotypes and Information polymorphism index mean was equal to 0.570 and 0.350 respectively. These markers were able to demonstrate three individual bands in UBC 125, 134 and 165 markers for Savannah, Olpro and Zarfam genotypes respectively. accordance obtained results from this study, peering  is showing that using of more diverse genotypes can be considered for breeding programs in order to exploit heterosis for producing of hybrid.
 

Keywords

References

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